Shotgun sphingolipid analysis of human aqueous humor

Anna Trzeciecka; Ulises Arbelo; Arturo Barron; Genea Edwards; Sruthi Sampathkumar; Carol Toris; Sanjoy K Bhattacharya

doi:10.1007/978-1-4939-7407-8_10

Shotgun sphingolipid analysis of human aqueous humor

Anna Trzeciecka, Ulises Arbelo, Arturo Barron, Genea Edwards, Sruthi Sampathkumar, Carol Toris, Sanjoy K Bhattacharya

Ophthalmology

Research output: Chapter in Book/Report/Conference proceeding › Chapter

2 Scopus citations

Abstract

This protocol provides a step-by-step guide to shotgun sphingolipid analysis of aqueous humor. We describe the Bligh and Dyer crude lipid extraction method and electrospray ionization tandem mass spectrometry (ESI-MS/MS) coupled with MZmine 2.21 data processing for identification and ratiometric quantitation of sphingosine, sphingosine-1-phosphate, sphingomyelin, and ceramide.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	97-107
Number of pages	11
Volume	1695
DOIs	https://doi.org/10.1007/978-1-4939-7407-8_10
State	Published - Jan 1 2018

Publication series

Name	Methods in Molecular Biology
Volume	1695
ISSN (Print)	1064-3745

Keywords

Aqueous humor
Ceramide
Electrospray ionization-tandem mass spectrometry (ESI-MS/MS)
MZmine 2.21
Shotgun lipidomics
Sphingolipids
Sphingomyelin
Sphingosine
Sphingosine-1-phosphate

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-4939-7407-8_10

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Cite this

Trzeciecka, A., Arbelo, U., Barron, A., Edwards, G., Sampathkumar, S., Toris, C., & Bhattacharya, S. K. (2018). Shotgun sphingolipid analysis of human aqueous humor. In Methods in Molecular Biology (Vol. 1695, pp. 97-107). (Methods in Molecular Biology; Vol. 1695). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7407-8_10

Shotgun sphingolipid analysis of human aqueous humor. / Trzeciecka, Anna; Arbelo, Ulises; Barron, Arturo; Edwards, Genea; Sampathkumar, Sruthi; Toris, Carol; Bhattacharya, Sanjoy K.

Methods in Molecular Biology. Vol. 1695 Humana Press Inc., 2018. p. 97-107 (Methods in Molecular Biology; Vol. 1695).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

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