@inbook{4c89c9fff6ea446cb815b469f6eacf02,
title = "Shotgun sphingolipid analysis of human aqueous humor",
abstract = "This protocol provides a step-by-step guide to shotgun sphingolipid analysis of aqueous humor. We describe the Bligh and Dyer crude lipid extraction method and electrospray ionization tandem mass spectrometry (ESI-MS/MS) coupled with MZmine 2.21 data processing for identification and ratiometric quantitation of sphingosine, sphingosine-1-phosphate, sphingomyelin, and ceramide.",
keywords = "Aqueous humor, Ceramide, Electrospray ionization-tandem mass spectrometry (ESI-MS/MS), MZmine 2.21, Shotgun lipidomics, Sphingolipids, Sphingomyelin, Sphingosine, Sphingosine-1-phosphate",
author = "Anna Trzeciecka and Ulises Arbelo and Arturo Barron and Genea Edwards and Sruthi Sampathkumar and Carol Toris and Bhattacharya, {Sanjoy K.}",
note = "Publisher Copyright: {\textcopyright} 2018, Springer Science+Business Media LLC. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.",
year = "2018",
doi = "10.1007/978-1-4939-7407-8_10",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "97--107",
booktitle = "Methods in Molecular Biology",
}