Shotgun sphingolipid analysis of human aqueous humor

Anna Trzeciecka, Ulises Arbelo, Arturo Barron, Genea Edwards, Sruthi Sampathkumar, Carol Toris, Sanjoy K Bhattacharya

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations


This protocol provides a step-by-step guide to shotgun sphingolipid analysis of aqueous humor. We describe the Bligh and Dyer crude lipid extraction method and electrospray ionization tandem mass spectrometry (ESI-MS/MS) coupled with MZmine 2.21 data processing for identification and ratiometric quantitation of sphingosine, sphingosine-1-phosphate, sphingomyelin, and ceramide.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages11
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Aqueous humor
  • Ceramide
  • Electrospray ionization-tandem mass spectrometry (ESI-MS/MS)
  • MZmine 2.21
  • Shotgun lipidomics
  • Sphingolipids
  • Sphingomyelin
  • Sphingosine
  • Sphingosine-1-phosphate

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Trzeciecka, A., Arbelo, U., Barron, A., Edwards, G., Sampathkumar, S., Toris, C., & Bhattacharya, S. K. (2018). Shotgun sphingolipid analysis of human aqueous humor. In Methods in Molecular Biology (Vol. 1695, pp. 97-107). (Methods in Molecular Biology; Vol. 1695). Humana Press Inc..