Short-term regulation of insulin gene transcription by glucose

Barbara Leibiger, Tilo Moede, Thomas Schwarz, Graham R. Brown, Martin Köhler, Ingo B. Leibiger, Per Olof Berggren

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Whereas short-term regulation of insulin biosynthesis at the level of translation is well accepted, glucose-dependent transcriptional control is still believed to be a long-term effect occurring after more than 2 hr of glucose stimulation. Because pancreatic β cells are exposed to elevated glucose levels for minutes rather than hours after food uptake, we hypothesized the existence of a short-term transcriptional control. By studying the dynamics of newly synthesized (prepro)insulin RNA and by employing on-line monitoring of gene expression in single, insulin-producing cells, we were able to provide convincing evidence that insulin gene transcription indeed is affected by glucose within minutes. Exposure of insulinoma cells and isolated pancreatic islets to elevated glucose for only 15 min resulted in a 2-to 5-fold elevation in (prepro)insulin mRNA levels within 60-90 min. Similarly, insulin promoter-driven green fluorescent protein expression in single insulin-producing cells was significantly enhanced after transient glucose stimulation. Thus, short-term signaling, such as that involved in insulin secretion, also may regulate insulin gene transcription.

Original languageEnglish
Pages (from-to)9307-9312
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume95
Issue number16
DOIs
StatePublished - Aug 4 1998
Externally publishedYes

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Insulin
Glucose
Genes
Insulinoma
Gene Expression Profiling
Green Fluorescent Proteins
Islets of Langerhans
RNA
Food
Messenger RNA

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Leibiger, B., Moede, T., Schwarz, T., Brown, G. R., Köhler, M., Leibiger, I. B., & Berggren, P. O. (1998). Short-term regulation of insulin gene transcription by glucose. Proceedings of the National Academy of Sciences of the United States of America, 95(16), 9307-9312. https://doi.org/10.1073/pnas.95.16.9307

Short-term regulation of insulin gene transcription by glucose. / Leibiger, Barbara; Moede, Tilo; Schwarz, Thomas; Brown, Graham R.; Köhler, Martin; Leibiger, Ingo B.; Berggren, Per Olof.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, No. 16, 04.08.1998, p. 9307-9312.

Research output: Contribution to journalArticle

Leibiger, B, Moede, T, Schwarz, T, Brown, GR, Köhler, M, Leibiger, IB & Berggren, PO 1998, 'Short-term regulation of insulin gene transcription by glucose', Proceedings of the National Academy of Sciences of the United States of America, vol. 95, no. 16, pp. 9307-9312. https://doi.org/10.1073/pnas.95.16.9307
Leibiger, Barbara ; Moede, Tilo ; Schwarz, Thomas ; Brown, Graham R. ; Köhler, Martin ; Leibiger, Ingo B. ; Berggren, Per Olof. / Short-term regulation of insulin gene transcription by glucose. In: Proceedings of the National Academy of Sciences of the United States of America. 1998 ; Vol. 95, No. 16. pp. 9307-9312.
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