Short-term cytolytic mediators' expression in decidual lymphocytes is enhanced by interleukin-15

Natasa Strbo, Gordana Laskarin, Tatjana Bogovic Crncic, Koraljka Juretic Frankovic, Ivica Bedenicki, Vlatka Sotosek Tokmadzic, Ljiljana Randic, Philippe Le Bouteiller, Julie Tabiasco, Eckhard R. Podack, Daniel Rukavina

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


Problem: We investigated whether decidual adherent cells (DAC) and interleukin (IL)-15, in comparison to interleukin (IL)-2 affect cytolytic potential of first trimester decidual lymphocytes (DL). Method of study: Decidual mononuclear cells were obtained by enzymatic digestion and density gradient centrifugation. Non-adherent DL were collected after 2-hr adherence and cultured for 18 or 72 hr with: IL-15 (0.5-5 ng/mL), IL-2 (100-1000 U/mL) or both of these cytokines, DAC (ratio 3:1 and 1:1) or DAC and anti-IL-15 antibody. Perforin, Fas ligand (FasL) and granzyme B were detected at mRNA level in indicated culture conditions. Cytolytic activity of DL against K-562, P815 and P815-Fas was measured by 2-hr PKH-26 cytotoxicity assay. The dynamics of perforin protein and mRNA expression were measured in DL after a contact with K-562 targets. Results: Interleukin-15 enhanced perforin, FasL and granzyme B transcription after 18-hr culture and prevented perforin protein downregulation, observed after DL culture. IL-2 had similar effects. DAC sustained perforin expression in DL and anti-IL-15 monoclonal antibody abrogated this effect. DAC increased cytotoxicity of DL against K-562 which was mediated by IL-15. Conclusion: Interleukin-15, probably produced by DAC, upregulates cytolytic mediators' expression and perforin-mediated cytotoxicity of DL, with equal efficiency as high concentrations of IL-2.

Original languageEnglish (US)
Pages (from-to)217-225
Number of pages9
JournalAmerican Journal of Reproductive Immunology
Issue number3
StatePublished - Mar 1 2006


  • Cytotoxicity
  • Decidual NK cells
  • Fas ligand
  • Interleukin-15
  • Perforin

ASJC Scopus subject areas

  • Immunology
  • Obstetrics and Gynecology


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