Short-term cytolytic mediators' expression in decidual lymphocytes is enhanced by interleukin-15

Natasa Strbo, Gordana Laskarin, Tatjana Bogovic Crncic, Koraljka Juretic Frankovic, Ivica Bedenicki, Vlatka Sotosek Tokmadzic, Ljiljana Randic, Philippe Le Bouteiller, Julie Tabiasco, Eckhard R. Podack, Daniel Rukavina

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Problem: We investigated whether decidual adherent cells (DAC) and interleukin (IL)-15, in comparison to interleukin (IL)-2 affect cytolytic potential of first trimester decidual lymphocytes (DL). Method of study: Decidual mononuclear cells were obtained by enzymatic digestion and density gradient centrifugation. Non-adherent DL were collected after 2-hr adherence and cultured for 18 or 72 hr with: IL-15 (0.5-5 ng/mL), IL-2 (100-1000 U/mL) or both of these cytokines, DAC (ratio 3:1 and 1:1) or DAC and anti-IL-15 antibody. Perforin, Fas ligand (FasL) and granzyme B were detected at mRNA level in indicated culture conditions. Cytolytic activity of DL against K-562, P815 and P815-Fas was measured by 2-hr PKH-26 cytotoxicity assay. The dynamics of perforin protein and mRNA expression were measured in DL after a contact with K-562 targets. Results: Interleukin-15 enhanced perforin, FasL and granzyme B transcription after 18-hr culture and prevented perforin protein downregulation, observed after DL culture. IL-2 had similar effects. DAC sustained perforin expression in DL and anti-IL-15 monoclonal antibody abrogated this effect. DAC increased cytotoxicity of DL against K-562 which was mediated by IL-15. Conclusion: Interleukin-15, probably produced by DAC, upregulates cytolytic mediators' expression and perforin-mediated cytotoxicity of DL, with equal efficiency as high concentrations of IL-2.

Original languageEnglish
Pages (from-to)217-225
Number of pages9
JournalAmerican Journal of Reproductive Immunology
Volume55
Issue number3
DOIs
StatePublished - Mar 1 2006
Externally publishedYes

Fingerprint

Interleukin-15
Perforin
Lymphocytes
Interleukin-2
Granzymes
Fas Ligand Protein
Messenger RNA
Density Gradient Centrifugation
First Pregnancy Trimester
Digestion
Proteins
Up-Regulation
Down-Regulation
Monoclonal Antibodies
Cytokines
Antibodies

Keywords

  • Cytotoxicity
  • Decidual NK cells
  • Fas ligand
  • Interleukin-15
  • Perforin

ASJC Scopus subject areas

  • Immunology
  • Obstetrics and Gynecology

Cite this

Short-term cytolytic mediators' expression in decidual lymphocytes is enhanced by interleukin-15. / Strbo, Natasa; Laskarin, Gordana; Crncic, Tatjana Bogovic; Frankovic, Koraljka Juretic; Bedenicki, Ivica; Tokmadzic, Vlatka Sotosek; Randic, Ljiljana; Le Bouteiller, Philippe; Tabiasco, Julie; Podack, Eckhard R.; Rukavina, Daniel.

In: American Journal of Reproductive Immunology, Vol. 55, No. 3, 01.03.2006, p. 217-225.

Research output: Contribution to journalArticle

Strbo, N, Laskarin, G, Crncic, TB, Frankovic, KJ, Bedenicki, I, Tokmadzic, VS, Randic, L, Le Bouteiller, P, Tabiasco, J, Podack, ER & Rukavina, D 2006, 'Short-term cytolytic mediators' expression in decidual lymphocytes is enhanced by interleukin-15', American Journal of Reproductive Immunology, vol. 55, no. 3, pp. 217-225. https://doi.org/10.1111/j.1600-0897.2005.00351.x
Strbo, Natasa ; Laskarin, Gordana ; Crncic, Tatjana Bogovic ; Frankovic, Koraljka Juretic ; Bedenicki, Ivica ; Tokmadzic, Vlatka Sotosek ; Randic, Ljiljana ; Le Bouteiller, Philippe ; Tabiasco, Julie ; Podack, Eckhard R. ; Rukavina, Daniel. / Short-term cytolytic mediators' expression in decidual lymphocytes is enhanced by interleukin-15. In: American Journal of Reproductive Immunology. 2006 ; Vol. 55, No. 3. pp. 217-225.
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abstract = "Problem: We investigated whether decidual adherent cells (DAC) and interleukin (IL)-15, in comparison to interleukin (IL)-2 affect cytolytic potential of first trimester decidual lymphocytes (DL). Method of study: Decidual mononuclear cells were obtained by enzymatic digestion and density gradient centrifugation. Non-adherent DL were collected after 2-hr adherence and cultured for 18 or 72 hr with: IL-15 (0.5-5 ng/mL), IL-2 (100-1000 U/mL) or both of these cytokines, DAC (ratio 3:1 and 1:1) or DAC and anti-IL-15 antibody. Perforin, Fas ligand (FasL) and granzyme B were detected at mRNA level in indicated culture conditions. Cytolytic activity of DL against K-562, P815 and P815-Fas was measured by 2-hr PKH-26 cytotoxicity assay. The dynamics of perforin protein and mRNA expression were measured in DL after a contact with K-562 targets. Results: Interleukin-15 enhanced perforin, FasL and granzyme B transcription after 18-hr culture and prevented perforin protein downregulation, observed after DL culture. IL-2 had similar effects. DAC sustained perforin expression in DL and anti-IL-15 monoclonal antibody abrogated this effect. DAC increased cytotoxicity of DL against K-562 which was mediated by IL-15. Conclusion: Interleukin-15, probably produced by DAC, upregulates cytolytic mediators' expression and perforin-mediated cytotoxicity of DL, with equal efficiency as high concentrations of IL-2.",
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T1 - Short-term cytolytic mediators' expression in decidual lymphocytes is enhanced by interleukin-15

AU - Strbo, Natasa

AU - Laskarin, Gordana

AU - Crncic, Tatjana Bogovic

AU - Frankovic, Koraljka Juretic

AU - Bedenicki, Ivica

AU - Tokmadzic, Vlatka Sotosek

AU - Randic, Ljiljana

AU - Le Bouteiller, Philippe

AU - Tabiasco, Julie

AU - Podack, Eckhard R.

AU - Rukavina, Daniel

PY - 2006/3/1

Y1 - 2006/3/1

N2 - Problem: We investigated whether decidual adherent cells (DAC) and interleukin (IL)-15, in comparison to interleukin (IL)-2 affect cytolytic potential of first trimester decidual lymphocytes (DL). Method of study: Decidual mononuclear cells were obtained by enzymatic digestion and density gradient centrifugation. Non-adherent DL were collected after 2-hr adherence and cultured for 18 or 72 hr with: IL-15 (0.5-5 ng/mL), IL-2 (100-1000 U/mL) or both of these cytokines, DAC (ratio 3:1 and 1:1) or DAC and anti-IL-15 antibody. Perforin, Fas ligand (FasL) and granzyme B were detected at mRNA level in indicated culture conditions. Cytolytic activity of DL against K-562, P815 and P815-Fas was measured by 2-hr PKH-26 cytotoxicity assay. The dynamics of perforin protein and mRNA expression were measured in DL after a contact with K-562 targets. Results: Interleukin-15 enhanced perforin, FasL and granzyme B transcription after 18-hr culture and prevented perforin protein downregulation, observed after DL culture. IL-2 had similar effects. DAC sustained perforin expression in DL and anti-IL-15 monoclonal antibody abrogated this effect. DAC increased cytotoxicity of DL against K-562 which was mediated by IL-15. Conclusion: Interleukin-15, probably produced by DAC, upregulates cytolytic mediators' expression and perforin-mediated cytotoxicity of DL, with equal efficiency as high concentrations of IL-2.

AB - Problem: We investigated whether decidual adherent cells (DAC) and interleukin (IL)-15, in comparison to interleukin (IL)-2 affect cytolytic potential of first trimester decidual lymphocytes (DL). Method of study: Decidual mononuclear cells were obtained by enzymatic digestion and density gradient centrifugation. Non-adherent DL were collected after 2-hr adherence and cultured for 18 or 72 hr with: IL-15 (0.5-5 ng/mL), IL-2 (100-1000 U/mL) or both of these cytokines, DAC (ratio 3:1 and 1:1) or DAC and anti-IL-15 antibody. Perforin, Fas ligand (FasL) and granzyme B were detected at mRNA level in indicated culture conditions. Cytolytic activity of DL against K-562, P815 and P815-Fas was measured by 2-hr PKH-26 cytotoxicity assay. The dynamics of perforin protein and mRNA expression were measured in DL after a contact with K-562 targets. Results: Interleukin-15 enhanced perforin, FasL and granzyme B transcription after 18-hr culture and prevented perforin protein downregulation, observed after DL culture. IL-2 had similar effects. DAC sustained perforin expression in DL and anti-IL-15 monoclonal antibody abrogated this effect. DAC increased cytotoxicity of DL against K-562 which was mediated by IL-15. Conclusion: Interleukin-15, probably produced by DAC, upregulates cytolytic mediators' expression and perforin-mediated cytotoxicity of DL, with equal efficiency as high concentrations of IL-2.

KW - Cytotoxicity

KW - Decidual NK cells

KW - Fas ligand

KW - Interleukin-15

KW - Perforin

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