Abstract
A variety of teratogens induce the loss of postaxial forelimb structures when administered during midgestation to the mouse. Previous studies demonstrated that teratogen exposure is associated with a reduction in zone of polarizing activity (ZPA) -related polarizing activity without a noticeable loss of Shh expression. Herein, we quantitatively confirm that expression of Shh, Ptch1, and Gli3 are unaltered by teratogen exposure and demonstrate that sonic hedgehog (Shh) translation is unaffected. Examination of the polarizing response of host chick wings to teratogen-exposed ZPA tissue revealed an induced growth response and ectopic induction of Fgf4, Bmp2, Ptch1, and Gli1 expression similar to control ZPA tissue. Control ZPA tissue altered the fate of cells destined to die in the anterior necrotic zone, whereas cell death ensued in hosts receiving teratogen-exposed grafts. Immunohistochemical studies localized Shh protein in the mouse limb to the posterior mesoderm and overlying ectoderm. We postulate that teratogen exposure alters the ability of Shh to signal to the ectoderm and present microarray and reverse transcriptase-polymerase chain reaction data, indicating that Shh signaling could occur in the limb bud ectoderm.
| Original language | English |
|---|---|
| Pages (from-to) | 313-325 |
| Number of pages | 13 |
| Journal | Developmental Dynamics |
| Volume | 233 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jun 1 2005 |
| Externally published | Yes |
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Keywords
- ANZ
- Microarray
- Polarizing activity
- Shh
- ZPA
ASJC Scopus subject areas
- Developmental Biology
- Cell Biology
Cite this
Shh signaling in limb bud ectoderm : Potential role in teratogen-induced postaxial ectrodactyly. / Bell, Sheila M.; Schreiner, Claire M.; Goetz, John A.; Robbins, David J; Scott, William J.
In: Developmental Dynamics, Vol. 233, No. 2, 01.06.2005, p. 313-325.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Shh signaling in limb bud ectoderm
T2 - Potential role in teratogen-induced postaxial ectrodactyly
AU - Bell, Sheila M.
AU - Schreiner, Claire M.
AU - Goetz, John A.
AU - Robbins, David J
AU - Scott, William J.
PY - 2005/6/1
Y1 - 2005/6/1
N2 - A variety of teratogens induce the loss of postaxial forelimb structures when administered during midgestation to the mouse. Previous studies demonstrated that teratogen exposure is associated with a reduction in zone of polarizing activity (ZPA) -related polarizing activity without a noticeable loss of Shh expression. Herein, we quantitatively confirm that expression of Shh, Ptch1, and Gli3 are unaltered by teratogen exposure and demonstrate that sonic hedgehog (Shh) translation is unaffected. Examination of the polarizing response of host chick wings to teratogen-exposed ZPA tissue revealed an induced growth response and ectopic induction of Fgf4, Bmp2, Ptch1, and Gli1 expression similar to control ZPA tissue. Control ZPA tissue altered the fate of cells destined to die in the anterior necrotic zone, whereas cell death ensued in hosts receiving teratogen-exposed grafts. Immunohistochemical studies localized Shh protein in the mouse limb to the posterior mesoderm and overlying ectoderm. We postulate that teratogen exposure alters the ability of Shh to signal to the ectoderm and present microarray and reverse transcriptase-polymerase chain reaction data, indicating that Shh signaling could occur in the limb bud ectoderm.
AB - A variety of teratogens induce the loss of postaxial forelimb structures when administered during midgestation to the mouse. Previous studies demonstrated that teratogen exposure is associated with a reduction in zone of polarizing activity (ZPA) -related polarizing activity without a noticeable loss of Shh expression. Herein, we quantitatively confirm that expression of Shh, Ptch1, and Gli3 are unaltered by teratogen exposure and demonstrate that sonic hedgehog (Shh) translation is unaffected. Examination of the polarizing response of host chick wings to teratogen-exposed ZPA tissue revealed an induced growth response and ectopic induction of Fgf4, Bmp2, Ptch1, and Gli1 expression similar to control ZPA tissue. Control ZPA tissue altered the fate of cells destined to die in the anterior necrotic zone, whereas cell death ensued in hosts receiving teratogen-exposed grafts. Immunohistochemical studies localized Shh protein in the mouse limb to the posterior mesoderm and overlying ectoderm. We postulate that teratogen exposure alters the ability of Shh to signal to the ectoderm and present microarray and reverse transcriptase-polymerase chain reaction data, indicating that Shh signaling could occur in the limb bud ectoderm.
KW - ANZ
KW - Microarray
KW - Polarizing activity
KW - Shh
KW - ZPA
UR - http://www.scopus.com/inward/record.url?scp=19544377007&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=19544377007&partnerID=8YFLogxK
U2 - 10.1002/dvdy.20409
DO - 10.1002/dvdy.20409
M3 - Article
C2 - 15858818
AN - SCOPUS:19544377007
VL - 233
SP - 313
EP - 325
JO - Developmental Dynamics
JF - Developmental Dynamics
SN - 1058-8388
IS - 2
ER -