Sheep heart RNA stimulates myofibril formation and beating in cardiac mutant axolotl hearts in organ culture

Chi Zhang, Sherrie M. LaFrance, Sharon L. Lemanski, Xupei Huang, Dipak K. Dube, Larry F. Lemanski

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

In the Mexican axolotl, Ambystoma mexicanum, recessive mutant gene c, when homozygous, results in a failure of the heart to form sarcomeric myofibrils and contract normally. Previous studies have shown that purified RNA from normal anterior endoderm or from medium conditioned with anterior endoderm/pre-cardiac mesoderm has the capacity to rescue mutant hearts in organ culture. In the present study, RNA extracted from adult sheep heart was tested for its capacity to promote differentiation in the mutant axolotl hearts. Mutant hearts cultured in the presence of the sheep heart RNA in Steinberg's solution for 48 h displayed rhythmic contractions. Ultrastructural studies showed that the rescued mutant axolotl ventricular myocardial cells contained myofibrils of normal morphology. Mutant hearts cultured in Steinberg's solution alone did not beat throughout their lengths and myofibrils were not observable in the ventricles. Confocal microscopy confirmed the increase of Tropomyosin expression and formation of myofibrils in mutant hearts treated by sheep heart RNA. Thus, sheep heart RNA promotes myofibrillogenesis and the development of contractile function in embryonic cardiac mutant axolotl hearts.

Original languageEnglish (US)
Pages (from-to)419-427
Number of pages9
JournalAnatomy and Embryology
Volume206
Issue number6
DOIs
StatePublished - May 1 2003

Keywords

  • Cardiac mutant axolotl
  • Heart induction
  • Myofibrillogenesis
  • Organ culture
  • Sheep heart RNA

ASJC Scopus subject areas

  • Anatomy
  • Developmental Biology
  • Cell Biology
  • Embryology

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    Zhang, C., LaFrance, S. M., Lemanski, S. L., Huang, X., Dube, D. K., & Lemanski, L. F. (2003). Sheep heart RNA stimulates myofibril formation and beating in cardiac mutant axolotl hearts in organ culture. Anatomy and Embryology, 206(6), 419-427. https://doi.org/10.1007/s00429-002-0307-0