Sexual dimorphism in hypothalamic inflammation in the offspring of dams exposed to a diet rich in high fat and branched-chain amino acids

Marianna Sadagurski, Lucas Kniess Debarba, Joao Pedro Werneck-de-Castro, Abear Ali Awada, Tess A. Baker, Ernesto Bernal Mizrachi

Research output: Contribution to journalArticle

Abstract

Branched-chain amino acid (BCAAs: leucine, isoleucine, and valine) contribute to the development of obesity-associated insulin resistance in the context of consumption of a high-fat diet (HFD) in humans and rodents. Maternal diet is a major determinant of offspring health, and there is strong evidence that maternal HFD alters hypothalamic developmental programming and disrupts offspring energy homeostasis in rodents. In this study, we exposed pregnant and lactating C57BL/6JB female mice to either HFD, HFD with supplemented BCAA (HFD+BCAA), or standard diet (SC), and we studied offspring metabolic phenotypes. Both maternal HFD and HFD supplemented with BCAA had similar effect rendering the offspring metabolic imbalance and impairing their ability to cope with HFD when challenged during aging. The metabolic effects of HFD challenge were more profound in females, worsening female offspring ability to cope with an HFD challenge by activating hypothalamic inflammation in aging. Moreover, the sex differences in hypothalamic estrogen receptor α (ER-α) expression levels were lost in female offspring upon HFD challenge, supporting a link between ER-α levels and hypothalamic inflammation in offspring and highlighting the programming potential of hypothalamic inflammatory responses and maternal nutrition.

Original languageEnglish (US)
Pages (from-to)E526-E534
JournalAmerican journal of physiology. Endocrinology and metabolism
Volume317
Issue number3
DOIs
StatePublished - Sep 1 2019

Fingerprint

Branched Chain Amino Acids
High Fat Diet
Sex Characteristics
Fats
Diet
Inflammation
Mothers
Estrogen Receptors
Rodentia
Isoleucine
Valine
Leucine
Insulin Resistance
Homeostasis
Obesity

Keywords

  • BCAA
  • hypothalamic inflammation
  • maternal nutrition
  • metabolic reprogramming

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

Cite this

Sexual dimorphism in hypothalamic inflammation in the offspring of dams exposed to a diet rich in high fat and branched-chain amino acids. / Sadagurski, Marianna; Debarba, Lucas Kniess; Werneck-de-Castro, Joao Pedro; Ali Awada, Abear; Baker, Tess A.; Bernal Mizrachi, Ernesto.

In: American journal of physiology. Endocrinology and metabolism, Vol. 317, No. 3, 01.09.2019, p. E526-E534.

Research output: Contribution to journalArticle

Sadagurski, Marianna ; Debarba, Lucas Kniess ; Werneck-de-Castro, Joao Pedro ; Ali Awada, Abear ; Baker, Tess A. ; Bernal Mizrachi, Ernesto. / Sexual dimorphism in hypothalamic inflammation in the offspring of dams exposed to a diet rich in high fat and branched-chain amino acids. In: American journal of physiology. Endocrinology and metabolism. 2019 ; Vol. 317, No. 3. pp. E526-E534.
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