Serum‐free growth of HTC cells containing glucocorticoid‐ and insulin‐inducible tyrosine aminotransferase and cytoplasmic glucocorticoid receptors

E. Brad Thompson, C. U. Anderson, M. E. Lippman

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HTC cells have been made to grow in chemically defined medium without any macromolecular supplements whatsoever. Initial estimates of their relative amino acid requirements have been made. The cells grown in the defined medium retain many of the differentiated features which have been the focus of investigation in their serum grown counterparts. Thus, the cells in defined medium contain cytoplasmic glucocorticoid receptors and have tyrosine aminotransferase which can be induced by glucocorticoids, serum or insulin. These cells also produce, in small amounts, an as yet undefined rat serum protein.

Original languageEnglish (US)
Pages (from-to)403-411
Number of pages9
JournalJournal of Cellular Physiology
Issue number1 S
StatePublished - Oct 1975


ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

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