Serum free growth of HTC cells containing glucocorticoid and insulin inducible tyrosine aminotransferase and cytoplasmic glucocorticoid receptors

E. B. Thompson, C. U. Anderson, Marc E Lippman

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

HTC cells have been made to grow in chemically defined medium without any macromolecular supplements whatsoever. Initial estimates of their relative amino acid requirements have been made. The cells grown in the defined medium retain many of the differentiated features which have been the focus of investigation in their serum grown counterparts. Thus, the cells in defined medium contain cytoplasmic glucocorticoid receptors and have tyrosine aminotransferase which can be induced by glucocorticoids, serum or insulin. These cells also produce, in small amounts, an as yet undefined rat serum protein.

Original languageEnglish
Pages (from-to)403-411
Number of pages9
JournalJournal of Cellular Physiology
Volume86
Issue number2 II
StatePublished - Dec 1 1975
Externally publishedYes

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Tyrosine Transaminase
Glucocorticoid Receptors
Cytoplasmic and Nuclear Receptors
Glucocorticoids
Rats
Blood Proteins
Cells
Insulin
Amino Acids
Growth
Serum

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Serum free growth of HTC cells containing glucocorticoid and insulin inducible tyrosine aminotransferase and cytoplasmic glucocorticoid receptors. / Thompson, E. B.; Anderson, C. U.; Lippman, Marc E.

In: Journal of Cellular Physiology, Vol. 86, No. 2 II, 01.12.1975, p. 403-411.

Research output: Contribution to journalArticle

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