Serum factor supporting long-term survival of rat central neurons in culture

Lawrence M. Kaufman, John N. Barrett

Research output: Contribution to journalArticle

94 Scopus citations

Abstract

Gel filtration of serum at pH 3.6 yielded a fraction that supported long-term (months) survival of dissociated rat central neurons in monolayer culture more reliably than the traditionally used unfractionated serum. The cultures remained neuron-rich, because this fraction did not support the proliferation of glia and fibroblasts that occurs in whole serum. With an apparent molecular weight of 55,000 and an isoelectric point of 5.6, the active factor (or factors) in this fraction is distinct from any well-defined growth factor.

Original languageEnglish (US)
Pages (from-to)1394-1396
Number of pages3
JournalScience
Volume220
Issue number4604
DOIs
StatePublished - Jan 1 1983

    Fingerprint

ASJC Scopus subject areas

  • General

Cite this