Serum cardiac troponin T as a biomarker for acute myocardial injury induced by low doses of isoproterenol in rats

Eugene Herman, Jun Zhang, Alan Knapton, Steven E Lipshultz, Nader Rifai, Frank Sistare

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In rats, high doses of isoproterenol (Iso) have caused acute myocardial lesions and increased serum levels of cardiac troponin T (cTnT). We determined whether low doses of Iso also cause cardiac alterations and whether monitoring cTnT levels could detect this injury. Rats received 8 to 500 μg/kg Iso and were followed for 3 to 48 h. Lesion severity was scored from 0 to 5. Within 3 h, mean cTnT was elevated in all 29 rats receiving 8, 16, 32, or 64 μg/kg Iso (0.20 to 0.28 ng/mL ), but minimal lesions occurred in only two animals. However, by 6 h, cardiac lesions and increases in serum cTnT (mean ≤ 0.21 to 0.23 ng/mL) were observed in all 14 rats receiving 32 or 64 μg/kg Iso. Doses of 125, 250, or 500 μg/kg Iso caused more significant increases in cTnT levels and marked myocardial lesions that reached a peak 3 to 6 h after dosing. The magnitude of the lesions and cTnT levels declined between 12 and 48 h post treatment. Thus, a range of low Iso doses can cause myocardial lesions, and serum cTnT levels can be monitored to detect the onset and progression of this type of acute cardiac injury in rats; however, careful attention to dosing and sampling times is critical to interpretation.

Original languageEnglish
Pages (from-to)211-221
Number of pages11
JournalCardiovascular Toxicology
Volume6
Issue number3-4
StatePublished - Sep 1 2006

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Troponin T
Biomarkers
Isoproterenol
Rats
Wounds and Injuries
Serum
Animals
Sampling
Monitoring

Keywords

  • Cardiac troponin T
  • Isoproterenol
  • Low dose
  • Myocardial damage

ASJC Scopus subject areas

  • Toxicology
  • Cardiology and Cardiovascular Medicine

Cite this

Herman, E., Zhang, J., Knapton, A., Lipshultz, S. E., Rifai, N., & Sistare, F. (2006). Serum cardiac troponin T as a biomarker for acute myocardial injury induced by low doses of isoproterenol in rats. Cardiovascular Toxicology, 6(3-4), 211-221.

Serum cardiac troponin T as a biomarker for acute myocardial injury induced by low doses of isoproterenol in rats. / Herman, Eugene; Zhang, Jun; Knapton, Alan; Lipshultz, Steven E; Rifai, Nader; Sistare, Frank.

In: Cardiovascular Toxicology, Vol. 6, No. 3-4, 01.09.2006, p. 211-221.

Research output: Contribution to journalArticle

Herman, E, Zhang, J, Knapton, A, Lipshultz, SE, Rifai, N & Sistare, F 2006, 'Serum cardiac troponin T as a biomarker for acute myocardial injury induced by low doses of isoproterenol in rats', Cardiovascular Toxicology, vol. 6, no. 3-4, pp. 211-221.
Herman E, Zhang J, Knapton A, Lipshultz SE, Rifai N, Sistare F. Serum cardiac troponin T as a biomarker for acute myocardial injury induced by low doses of isoproterenol in rats. Cardiovascular Toxicology. 2006 Sep 1;6(3-4):211-221.
Herman, Eugene ; Zhang, Jun ; Knapton, Alan ; Lipshultz, Steven E ; Rifai, Nader ; Sistare, Frank. / Serum cardiac troponin T as a biomarker for acute myocardial injury induced by low doses of isoproterenol in rats. In: Cardiovascular Toxicology. 2006 ; Vol. 6, No. 3-4. pp. 211-221.
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