Serum amyloid A and plasma protein electrophoresis fractions in farmed white-tailed deer

Carolyn Cray, Roxanne I. Knibb, Jeffrey R. Knibb

Research output: Contribution to journalArticle

Abstract

Tools to measure the acute-phase response have been utilized widely in veterinary medicine. Evaluation by plasma protein electrophoresis (PPEP) has become an increasingly common assay in veterinary clinical pathology. Commercial reagents for serum amyloid A (SAA) have been validated for use in a variety of wildlife species. We analyzed samples from 29 healthy fawns and 60 healthy adult farmed white-tailed deer (WTD; Odocoileus virginianus) using an automated assay for SAA and a semi-automated method for PPEP. The robust statistical method for reference interval generation was used. SAA levels in fawns (0.1–26 mg/L) were found to be significantly higher than those in adults (0.1–5 mg/L, p < 0.01). The mean total protein was significantly lower in fawns (48 ± 10 g/L, p < 0.01) than in adults (73 ±5 g/L). The albumin-to-globulin ratio was also lower in fawns (0.56 ± 0.14) than in adults (1.25 ± 0.19, p < 0.01). Changes in SAA levels were observed in a variety of clinically abnormal animals. The combined use of the automated and semi-automated assays in our study may provide an additional valuable assessment tool in the care of captive WTD populations, for research studies, and for monitoring free-ranging animals.

Original languageEnglish (US)
Pages (from-to)458-462
Number of pages5
JournalJournal of Veterinary Diagnostic Investigation
Volume31
Issue number3
DOIs
StatePublished - May 1 2019

Keywords

  • Odocoileus virginianus
  • acute-phase protein
  • albumin:globulin ratio
  • plasma protein electrophoresis
  • reference intervals
  • serum amyloid A
  • white-tailed deer

ASJC Scopus subject areas

  • veterinary(all)

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