Serum amyloid A and plasma protein electrophoresis fractions in farmed white-tailed deer

Carolyn Cray, Roxanne I. Knibb, Jeffrey R. Knibb

Research output: Contribution to journalArticle

Abstract

Tools to measure the acute-phase response have been utilized widely in veterinary medicine. Evaluation by plasma protein electrophoresis (PPEP) has become an increasingly common assay in veterinary clinical pathology. Commercial reagents for serum amyloid A (SAA) have been validated for use in a variety of wildlife species. We analyzed samples from 29 healthy fawns and 60 healthy adult farmed white-tailed deer (WTD; Odocoileus virginianus) using an automated assay for SAA and a semi-automated method for PPEP. The robust statistical method for reference interval generation was used. SAA levels in fawns (0.1–26 mg/L) were found to be significantly higher than those in adults (0.1–5 mg/L, p < 0.01). The mean total protein was significantly lower in fawns (48 ± 10 g/L, p < 0.01) than in adults (73 ±5 g/L). The albumin-to-globulin ratio was also lower in fawns (0.56 ± 0.14) than in adults (1.25 ± 0.19, p < 0.01). Changes in SAA levels were observed in a variety of clinically abnormal animals. The combined use of the automated and semi-automated assays in our study may provide an additional valuable assessment tool in the care of captive WTD populations, for research studies, and for monitoring free-ranging animals.

Original languageEnglish (US)
JournalJournal of Veterinary Diagnostic Investigation
DOIs
StatePublished - Jan 1 2019

Fingerprint

Serum Amyloid A Protein
fawns
Deer
Odocoileus virginianus
amyloid
blood proteins
electrophoresis
Electrophoresis
Blood Proteins
assays
Veterinary Pathology
Acute-Phase Reaction
Clinical Pathology
Veterinary Medicine
Globulins
globulins
veterinary medicine
albumins
Albumins
wildlife

Keywords

  • Acute-phase protein
  • albumin:globulin ratio
  • Odocoileus virginianus
  • plasma protein electrophoresis
  • reference intervals
  • serum amyloid A
  • white-tailed deer

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Serum amyloid A and plasma protein electrophoresis fractions in farmed white-tailed deer. / Cray, Carolyn; Knibb, Roxanne I.; Knibb, Jeffrey R.

In: Journal of Veterinary Diagnostic Investigation, 01.01.2019.

Research output: Contribution to journalArticle

@article{22b526b26e854d98b9f7e685a5864fd6,
title = "Serum amyloid A and plasma protein electrophoresis fractions in farmed white-tailed deer",
abstract = "Tools to measure the acute-phase response have been utilized widely in veterinary medicine. Evaluation by plasma protein electrophoresis (PPEP) has become an increasingly common assay in veterinary clinical pathology. Commercial reagents for serum amyloid A (SAA) have been validated for use in a variety of wildlife species. We analyzed samples from 29 healthy fawns and 60 healthy adult farmed white-tailed deer (WTD; Odocoileus virginianus) using an automated assay for SAA and a semi-automated method for PPEP. The robust statistical method for reference interval generation was used. SAA levels in fawns (0.1–26 mg/L) were found to be significantly higher than those in adults (0.1–5 mg/L, p < 0.01). The mean total protein was significantly lower in fawns (48 ± 10 g/L, p < 0.01) than in adults (73 ±5 g/L). The albumin-to-globulin ratio was also lower in fawns (0.56 ± 0.14) than in adults (1.25 ± 0.19, p < 0.01). Changes in SAA levels were observed in a variety of clinically abnormal animals. The combined use of the automated and semi-automated assays in our study may provide an additional valuable assessment tool in the care of captive WTD populations, for research studies, and for monitoring free-ranging animals.",
keywords = "Acute-phase protein, albumin:globulin ratio, Odocoileus virginianus, plasma protein electrophoresis, reference intervals, serum amyloid A, white-tailed deer",
author = "Carolyn Cray and Knibb, {Roxanne I.} and Knibb, {Jeffrey R.}",
year = "2019",
month = "1",
day = "1",
doi = "10.1177/1040638719836150",
language = "English (US)",
journal = "Journal of Veterinary Diagnostic Investigation",
issn = "1040-6387",
publisher = "American Association of Veterinary Laboratory Diagnosticians",

}

TY - JOUR

T1 - Serum amyloid A and plasma protein electrophoresis fractions in farmed white-tailed deer

AU - Cray, Carolyn

AU - Knibb, Roxanne I.

AU - Knibb, Jeffrey R.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Tools to measure the acute-phase response have been utilized widely in veterinary medicine. Evaluation by plasma protein electrophoresis (PPEP) has become an increasingly common assay in veterinary clinical pathology. Commercial reagents for serum amyloid A (SAA) have been validated for use in a variety of wildlife species. We analyzed samples from 29 healthy fawns and 60 healthy adult farmed white-tailed deer (WTD; Odocoileus virginianus) using an automated assay for SAA and a semi-automated method for PPEP. The robust statistical method for reference interval generation was used. SAA levels in fawns (0.1–26 mg/L) were found to be significantly higher than those in adults (0.1–5 mg/L, p < 0.01). The mean total protein was significantly lower in fawns (48 ± 10 g/L, p < 0.01) than in adults (73 ±5 g/L). The albumin-to-globulin ratio was also lower in fawns (0.56 ± 0.14) than in adults (1.25 ± 0.19, p < 0.01). Changes in SAA levels were observed in a variety of clinically abnormal animals. The combined use of the automated and semi-automated assays in our study may provide an additional valuable assessment tool in the care of captive WTD populations, for research studies, and for monitoring free-ranging animals.

AB - Tools to measure the acute-phase response have been utilized widely in veterinary medicine. Evaluation by plasma protein electrophoresis (PPEP) has become an increasingly common assay in veterinary clinical pathology. Commercial reagents for serum amyloid A (SAA) have been validated for use in a variety of wildlife species. We analyzed samples from 29 healthy fawns and 60 healthy adult farmed white-tailed deer (WTD; Odocoileus virginianus) using an automated assay for SAA and a semi-automated method for PPEP. The robust statistical method for reference interval generation was used. SAA levels in fawns (0.1–26 mg/L) were found to be significantly higher than those in adults (0.1–5 mg/L, p < 0.01). The mean total protein was significantly lower in fawns (48 ± 10 g/L, p < 0.01) than in adults (73 ±5 g/L). The albumin-to-globulin ratio was also lower in fawns (0.56 ± 0.14) than in adults (1.25 ± 0.19, p < 0.01). Changes in SAA levels were observed in a variety of clinically abnormal animals. The combined use of the automated and semi-automated assays in our study may provide an additional valuable assessment tool in the care of captive WTD populations, for research studies, and for monitoring free-ranging animals.

KW - Acute-phase protein

KW - albumin:globulin ratio

KW - Odocoileus virginianus

KW - plasma protein electrophoresis

KW - reference intervals

KW - serum amyloid A

KW - white-tailed deer

UR - http://www.scopus.com/inward/record.url?scp=85062868522&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85062868522&partnerID=8YFLogxK

U2 - 10.1177/1040638719836150

DO - 10.1177/1040638719836150

M3 - Article

C2 - 30852951

AN - SCOPUS:85062868522

JO - Journal of Veterinary Diagnostic Investigation

JF - Journal of Veterinary Diagnostic Investigation

SN - 1040-6387

ER -