Serum albumin in 2D: A Langmuir monolayer approach

Nicholas F. Crawford, Roger Leblanc

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Understanding of protein interaction at the molecular level raises certain difficulties which is the reason a model membrane system such as the Langmuir monolayer technique was developed. Ubiquitous proteins such as serum albumin comprise 50% of human blood plasma protein content and are involved in many biological functions. The important nature of this class of protein demands that it be studied in detail while modifying the experimental conditions in two dimensions to observe it in all types of environments. While different from bulk colloidal solution work, the two dimensional approach allows for the observation of the interaction between molecules and subphase at the air-water interface. Compiled in this review are studies which highlight the characterization of this protein using various surroundings and also observing the types of interactions it would have when at the biomembrane interface. Free-energy changes between molecules, packing status of the bulk analyte at the interface as well as phase transitions as the monolayer forms a more organized or aggregated state are just some of the characteristics which are observed through the Langmuir technique. This unique methodology demonstrates the chemical behavior and physical behavior of this protein at the phase boundary throughout the compression of the monolayer.

Original languageEnglish
Pages (from-to)131-138
Number of pages8
JournalAdvances in Colloid and Interface Science
Volume207
Issue number1
DOIs
StatePublished - Jan 1 2014

Fingerprint

monomolecular films
albumins
Serum Albumin
serums
Monolayers
proteins
Proteins
Molecules
blood plasma
Phase boundaries
Free energy
interactions
Blood Proteins
Blood
Phase transitions
molecules
Membranes
free energy
methodology
Water

Keywords

  • Bovine serum albumin
  • Human serum albumin
  • Langmuir monolayer
  • Langmuir-Blodgett films
  • Mixed monolayers

ASJC Scopus subject areas

  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

Cite this

Serum albumin in 2D : A Langmuir monolayer approach. / Crawford, Nicholas F.; Leblanc, Roger.

In: Advances in Colloid and Interface Science, Vol. 207, No. 1, 01.01.2014, p. 131-138.

Research output: Contribution to journalArticle

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