Serologic identification and characterization of a macaque T-lymphotropic retrovirus closely related to HTLV-III

P. J. Kanki, M. F. McLane, N. W. King, N. L. Letvin, R. D. Hunt, P. Sehgal, M. D. Daniel, Ronald Charles Desrosiers, M. Essex

Research output: Contribution to journalArticle

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Abstract

Human T-lymphotropic virus type III (HTLV-III) is thought to play an etiologic role in the development of the acquired immune deficiency syndrome (AIDS). In this study the serologic characterization of a new simian retrovirus that is related to HTLV-III is described. This new virus, here referred to as STLV-III, was isolated from sick macaques at the New England Regional Primate Research Center. Radioimmunoprecipitation analysis revealed STLV-III-specific proteins of 160, 120, 55, and 24 kilodaltons, all similar in size to the major gag and env proteins of HTLV-III. These antigens were recognized by representative macaque serum samples and human reference serum samples positive for HTLV-III antibodies. Monoclonal antibodies directed to p24, the major core protein of HTLV-III, also immunoprecipitated a 24-kilodalton species in lysates of cells infected with the macaque virus. This HTLV-III-related virus, which naturally infects a nonhuman primate species, may provide a useful model for the study of HTLV-III and the pathogenesis of AIDS.

Original languageEnglish (US)
Pages (from-to)1199-1201
Number of pages3
JournalScience
Volume228
Issue number4704
DOIs
StatePublished - 1985
Externally publishedYes

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Macaca
Retroviridae
HIV
Viruses
Primates
Simian Retroviruses
Acquired Immunodeficiency Syndrome
Radioimmunoprecipitation Assay
env Gene Products
Viral Core Proteins
gag Gene Products
HIV Antibodies
New England
Serum
Monoclonal Antibodies
Antigens
Research
Proteins

ASJC Scopus subject areas

  • General

Cite this

Kanki, P. J., McLane, M. F., King, N. W., Letvin, N. L., Hunt, R. D., Sehgal, P., ... Essex, M. (1985). Serologic identification and characterization of a macaque T-lymphotropic retrovirus closely related to HTLV-III. Science, 228(4704), 1199-1201. https://doi.org/10.1126/science.3873705

Serologic identification and characterization of a macaque T-lymphotropic retrovirus closely related to HTLV-III. / Kanki, P. J.; McLane, M. F.; King, N. W.; Letvin, N. L.; Hunt, R. D.; Sehgal, P.; Daniel, M. D.; Desrosiers, Ronald Charles; Essex, M.

In: Science, Vol. 228, No. 4704, 1985, p. 1199-1201.

Research output: Contribution to journalArticle

Kanki, PJ, McLane, MF, King, NW, Letvin, NL, Hunt, RD, Sehgal, P, Daniel, MD, Desrosiers, RC & Essex, M 1985, 'Serologic identification and characterization of a macaque T-lymphotropic retrovirus closely related to HTLV-III', Science, vol. 228, no. 4704, pp. 1199-1201. https://doi.org/10.1126/science.3873705
Kanki, P. J. ; McLane, M. F. ; King, N. W. ; Letvin, N. L. ; Hunt, R. D. ; Sehgal, P. ; Daniel, M. D. ; Desrosiers, Ronald Charles ; Essex, M. / Serologic identification and characterization of a macaque T-lymphotropic retrovirus closely related to HTLV-III. In: Science. 1985 ; Vol. 228, No. 4704. pp. 1199-1201.
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