Abstract
In psittacine birds, the diagnosis of sarcocystosis is usually made on postmortem examination, and antemortem testing has been limited to muscle biopsy. We studied the applications of a serologic assay for antibody and plasma protein electrophoresis for changes in protein fractions in the antemortem diagnosis of sarcocystosis in psittacine birds. Several confirmed cases of sarcocystosis in psittacine birds were examined. To detect antibody, an indirect immunofluorescent assay was employed using Sarcocystis falcatula merozoites. Plasma samples from birds that demonstrated chronic neurologic signs had reactivity to the S falcatula antigen, and increases were found in the beta and gamma fractions by plasma protein electrophoresis. Conversely, in birds with the classic peracute onset of dyspnea and subsequent death, test results by either methodology were negative. These findings support the use of these 2 diagnostic tools as aids in the antemortem diagnosis of the nonperacute form of sarcocystosis in psittacine birds.
| Original language | English |
|---|---|
| Pages (from-to) | 208-215 |
| Number of pages | 8 |
| Journal | Journal of Avian Medicine and Surgery |
| Volume | 19 |
| Issue number | 3 |
| DOIs | |
| State | Published - Sep 1 2005 |
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Keywords
- Antemortem
- Antibody
- Avian
- Immunofluorescence
- Protein electrophoresis
- Sarcocystis
- Serology
ASJC Scopus subject areas
- veterinary(all)
Cite this
Serologic diagnosis of sarcocystosis in psittacine birds : 16 cases. / Cray, Carolyn; Zielezienski-Roberts, Karen; Bonda, Michael; Stevenso, Rhoda; Ness, Robert; Clubb, Susan; Marsh, Antoinette.
In: Journal of Avian Medicine and Surgery, Vol. 19, No. 3, 01.09.2005, p. 208-215.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Serologic diagnosis of sarcocystosis in psittacine birds
T2 - 16 cases
AU - Cray, Carolyn
AU - Zielezienski-Roberts, Karen
AU - Bonda, Michael
AU - Stevenso, Rhoda
AU - Ness, Robert
AU - Clubb, Susan
AU - Marsh, Antoinette
PY - 2005/9/1
Y1 - 2005/9/1
N2 - In psittacine birds, the diagnosis of sarcocystosis is usually made on postmortem examination, and antemortem testing has been limited to muscle biopsy. We studied the applications of a serologic assay for antibody and plasma protein electrophoresis for changes in protein fractions in the antemortem diagnosis of sarcocystosis in psittacine birds. Several confirmed cases of sarcocystosis in psittacine birds were examined. To detect antibody, an indirect immunofluorescent assay was employed using Sarcocystis falcatula merozoites. Plasma samples from birds that demonstrated chronic neurologic signs had reactivity to the S falcatula antigen, and increases were found in the beta and gamma fractions by plasma protein electrophoresis. Conversely, in birds with the classic peracute onset of dyspnea and subsequent death, test results by either methodology were negative. These findings support the use of these 2 diagnostic tools as aids in the antemortem diagnosis of the nonperacute form of sarcocystosis in psittacine birds.
AB - In psittacine birds, the diagnosis of sarcocystosis is usually made on postmortem examination, and antemortem testing has been limited to muscle biopsy. We studied the applications of a serologic assay for antibody and plasma protein electrophoresis for changes in protein fractions in the antemortem diagnosis of sarcocystosis in psittacine birds. Several confirmed cases of sarcocystosis in psittacine birds were examined. To detect antibody, an indirect immunofluorescent assay was employed using Sarcocystis falcatula merozoites. Plasma samples from birds that demonstrated chronic neurologic signs had reactivity to the S falcatula antigen, and increases were found in the beta and gamma fractions by plasma protein electrophoresis. Conversely, in birds with the classic peracute onset of dyspnea and subsequent death, test results by either methodology were negative. These findings support the use of these 2 diagnostic tools as aids in the antemortem diagnosis of the nonperacute form of sarcocystosis in psittacine birds.
KW - Antemortem
KW - Antibody
KW - Avian
KW - Immunofluorescence
KW - Protein electrophoresis
KW - Sarcocystis
KW - Serology
UR - http://www.scopus.com/inward/record.url?scp=33845293798&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33845293798&partnerID=8YFLogxK
U2 - 10.1647/1082-6742(2005)019[0208:SDOSIP]2.0.CO;2
DO - 10.1647/1082-6742(2005)019[0208:SDOSIP]2.0.CO;2
M3 - Article
AN - SCOPUS:33845293798
VL - 19
SP - 208
EP - 215
JO - Journal of Avian Medicine and Surgery
JF - Journal of Avian Medicine and Surgery
SN - 1082-6742
IS - 3
ER -