SERIAL SECTION RECONSTRUCTIONS OF MOUSE TASTE BUDS USING HIGH VOLTAGE ELECTRON MICROSCOPY.

J. Kinnamon, R. Delay, S. Young, S. Roper

Research output: Contribution to journalConference article

Abstract

Using HVEM of serial sections and computer generated three-dimensional reconstructions, the authors are analyzing intercellular relationships, cellular morphology and patterns of synaptic connectivity in the vertebrate taste bud (TB). HVEM is especially suited for this; it is much easier to cut serial thick sections (0. 5-1. 0 mu m) than thin ( less than 0. 1 mu m) sections. With the combination of HVEM and the computer models of taste buds and taste cells in the mouse are generated more rapidly and efficiently than with serial reconstructions using conventional transmission electron microscopy (CTEM).

Original languageEnglish (US)
Pages (from-to)297-302
Number of pages6
JournalLawrence Berkeley Laboratory (Report) LBL
StatePublished - Dec 1 1983
Externally publishedYes

ASJC Scopus subject areas

  • Engineering(all)

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