Serial magnetic resonance imaging in experimental primate stroke: Validation of MRI for pre-clinical cerebroprotective trials

William J. Mack, Ricardo J. Komotar, J. Mocco, Alexander L. Coon, Daniel J. Hoh, Ryan G. King, Andrew F. Ducruet, Evan R. Ransom, Marcello Oppermann, Robert DeLaPaz, E. Sander Connolly

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Precise assessment of stroke outcome is critical for pre-clinical testing of cerebroprotective strategies. Differences in stroke volume measured by various magnetic resonance imaging (MRI) techniques are documented in humans, but not well described in experimental primate stroke. This study characterizes changes in stroke volume using serial MRI scans in a baboon model of reperfused cerebral ischemia. The location/area of hyperintensity on MRI corresponded with the TTC-stained infarct region. T2-weighted fast spin echo (T2W FSE), fluid attenuated inversion recovery (FLAIR), and diffusion weighted imaging (DWI) showed a decrease in infarct volume between 72 h and nine days post-ischemia (p = ns, p = 0.029, and p = 0.006). T2W FSE and FLAIR demonstrated an increase in infarct volume from 24 h to nine days post-ischemia, while DWI displayed a decrease over the same period. While early T2W FSE, FLAIR, and DWI all correlated with late infarct volume (p < 0.001), 72 h T2W FSE was the best direct measure (2.39% ± 1.40% unity deviation). Serial MRI in a nonhuman primate model of focal cerebral ischemia recapitulates findings in clinical stroke. MRI at 72 h accurately predicts late infarct volume.

Original languageEnglish (US)
Pages (from-to)846-852
Number of pages7
JournalNeurological Research
Volume25
Issue number8
DOIs
StatePublished - Dec 1 2003
Externally publishedYes

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Keywords

  • Magnetic resonance imaging
  • Primate model
  • Stroke

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Mack, W. J., Komotar, R. J., Mocco, J., Coon, A. L., Hoh, D. J., King, R. G., Ducruet, A. F., Ransom, E. R., Oppermann, M., DeLaPaz, R., & Connolly, E. S. (2003). Serial magnetic resonance imaging in experimental primate stroke: Validation of MRI for pre-clinical cerebroprotective trials. Neurological Research, 25(8), 846-852. https://doi.org/10.1179/016164103771953943