Serial analysis of microtubules in cultured rat sensory axons

Drnnis Bray, Mary B Bunge

Research output: Contribution to journalArticle

121 Citations (Scopus)

Abstract

A fascicle of axons in an organotypic culture of rat dorsal root ganglion was analysed in 190 serial transverse sections, a total fascicle length calculated to be 12.8μm. The number, position and continuity of microtubules and components of smooth endoplasmic reticulum (SER) were examined. The 187 microtubules traced in 27 axons showed a total of 32 terminations in the series; 14 of these were 'starts' and 18 were 'stops' in relation to progression away from the neuronal soma; three tubules began and ended in the series. Microtubules overall were long, their lengths calculated to average 108μm. Terminations usually occurred close to the plasma membrane but otherwise exhibited no obvious morphological specialization. Microtubules in some axons were concentrated in the periphery of the axon, a region from which neurofilaments were excluded. The relative position of tubules in the axon did not change in any systematic way (for example, by rotation) throughout the series. The SER showed marked variation in form, from the expected membrane-bounded vesicle to profiles that resembled a microtubule or a thinner densely stained filament.

Original languageEnglish
Pages (from-to)589-605
Number of pages17
JournalJournal of Neurocytology
Volume10
Issue number4
DOIs
StatePublished - Aug 1 1981
Externally publishedYes

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Microtubules
Axons
Smooth Endoplasmic Reticulum
Intermediate Filaments
Spinal Ganglia
Carisoprodol
Cell Membrane
Membranes

ASJC Scopus subject areas

  • Neuroscience(all)
  • Histology
  • Anatomy
  • Cell Biology

Cite this

Serial analysis of microtubules in cultured rat sensory axons. / Bray, Drnnis; Bunge, Mary B.

In: Journal of Neurocytology, Vol. 10, No. 4, 01.08.1981, p. 589-605.

Research output: Contribution to journalArticle

Bray, Drnnis ; Bunge, Mary B. / Serial analysis of microtubules in cultured rat sensory axons. In: Journal of Neurocytology. 1981 ; Vol. 10, No. 4. pp. 589-605.
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