Sequence analysis of the new human T cell leukemia/lymphoma virus type I isolate (HTLV-I) in Israel

Y. Kilim, Joseph D Rosenblatt, Y. L. Danon

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Recent studies have established the presence of human T cell leukemia/ lymphoma virus type I (HTLV-I) in Israel. The entire nucleotide sequence of HTLV-I virus from a previously described HE isolate of a Mashadi Jewish Iranian patient was determined. To further characterize the LTR and env genes from the HTLV-I isolate we employed polymerase chain reaction amplification with subsequent cloning and sequencing of the amplified products on both strands. Sequence analyses of amplified LTR regions of this variant showed marked nucleotide homology of 98% compared to Japanese isolates, while African and Indo-Malay (Papua, New Guinea) and Solomon Island isolates showed more divergence with sequence homology of 95% and 91%. Higher homology of 98-99% was conserved in the amplified HTLV-env gene. In this respect the Iranian isolate was most similar to the African and Japanese isolate and divergent from the Melanesian HTLV-I variant, supporting the theory that HTLV-I may have originated in Africa and reached the Far East by overland trade routes.

Original languageEnglish
Pages (from-to)891-894
Number of pages4
JournalIsrael Journal of Medical Sciences
Volume30
Issue number12
StatePublished - Dec 1 1994
Externally publishedYes

Fingerprint

Deltaretrovirus
Human T-lymphotropic virus 1
T-cells
Israel
Nucleotides
Viruses
Sequence Analysis
Genes
Cloning
Polymerase chain reaction
env Genes
Amplification
Melanesia
Papua New Guinea
Far East
Sequence Homology
Organism Cloning
Polymerase Chain Reaction

Keywords

  • DNA sequence
  • env
  • HTLV-I
  • LTR region

ASJC Scopus subject areas

  • Bioengineering
  • Medicine(all)

Cite this

Sequence analysis of the new human T cell leukemia/lymphoma virus type I isolate (HTLV-I) in Israel. / Kilim, Y.; Rosenblatt, Joseph D; Danon, Y. L.

In: Israel Journal of Medical Sciences, Vol. 30, No. 12, 01.12.1994, p. 891-894.

Research output: Contribution to journalArticle

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