Sequence analysis of the diabetes-protective human leukocyte antigen-DQB1*0602 allele in unaffected, islet cell antibody-positive first degree relatives and in rare patients with type 1 diabetes

Alberto Pugliese, Eiji Kawasaki, Markus Zeller, Liping Yu, Sunanda Babu, Michele Solimena, Carlos T Moraes, Massimo Pietropaolo, Robert P. Friday, Massimo Trucco, Camillo Ricordi, Marie Allen, Janelle A. Noble, Henry A. Erlich, George S. Eisenbarth

Research output: Contribution to journalArticle

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Abstract

The human leukocyte antigen (HLA)-DQA1*0102/DQB1*0602/ DRBl*1501 (DR2) haplotype confers strong protection from type 1 diabetes. Growing evidence suggests that such protection may be mostly encoded by the DQB1*0602 allele, and we reported that even first degree relatives with islet cell antibodies (ICA) have an extremely low diabetes risk if they carry DQB1*0602. Recently, novel variants of the DQB1*0602 and *0603 alleles were reported in four patients with type i diabetes originally typed as DQB1*0602 with conventional techniques. One inference from this observation is that DQB1*0602 may confer absolute protection and may never occur in type i diabetes. By this hypothesis, all patients typed as DQB1*0602 positive with conventional techniques should carry one of the above diabetes-permissive variants instead of the protective DQB1*0602. Such variants could also occur in ICA/DQB1*0602-positive relatives, with the implication that their diabetes risk could be significantly higher than previously estimated. We therefore sequenced the DQB1*0602 and DQA1*0102 alleles in all ICA/DQB1*0602-positive relatives (n = 8) previously described and in six rare patients with type i diabetes and DQB1*0602. We found that all relatives and patients carry the known DQB1*0602 and DQA1*0102 sequences, and none of them has the mtDNA A3243G mutation associated with late-onset diabetes in ICA-positive individuals. These findings suggest that diabetes-permissive DQB1*0602/3 variants may be very rare. Thus, although the protective effect associated with DQB1*0602 is extremely powerful, it is not absolute. Nonetheless, the development of diabetes in individuals with DQB1*0602 remains extremely unlikely, even in the presence of ICA, as confirmed by our further evaluation of ICA/DQB1*0602-positive relatives, none of whom has yet developed diabetes.

Original languageEnglish
Pages (from-to)1722-1728
Number of pages7
JournalJournal of Clinical Endocrinology and Metabolism
Volume84
Issue number5
StatePublished - Dec 1 1999

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Medical problems
HLA Antigens
Type 1 Diabetes Mellitus
Sequence Analysis
Alleles
HLA-DQB1 antigen
islet cell antibody
Mitochondrial DNA
Haplotypes

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Sequence analysis of the diabetes-protective human leukocyte antigen-DQB1*0602 allele in unaffected, islet cell antibody-positive first degree relatives and in rare patients with type 1 diabetes. / Pugliese, Alberto; Kawasaki, Eiji; Zeller, Markus; Yu, Liping; Babu, Sunanda; Solimena, Michele; Moraes, Carlos T; Pietropaolo, Massimo; Friday, Robert P.; Trucco, Massimo; Ricordi, Camillo; Allen, Marie; Noble, Janelle A.; Erlich, Henry A.; Eisenbarth, George S.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 84, No. 5, 01.12.1999, p. 1722-1728.

Research output: Contribution to journalArticle

Pugliese, A, Kawasaki, E, Zeller, M, Yu, L, Babu, S, Solimena, M, Moraes, CT, Pietropaolo, M, Friday, RP, Trucco, M, Ricordi, C, Allen, M, Noble, JA, Erlich, HA & Eisenbarth, GS 1999, 'Sequence analysis of the diabetes-protective human leukocyte antigen-DQB1*0602 allele in unaffected, islet cell antibody-positive first degree relatives and in rare patients with type 1 diabetes', Journal of Clinical Endocrinology and Metabolism, vol. 84, no. 5, pp. 1722-1728.
Pugliese, Alberto ; Kawasaki, Eiji ; Zeller, Markus ; Yu, Liping ; Babu, Sunanda ; Solimena, Michele ; Moraes, Carlos T ; Pietropaolo, Massimo ; Friday, Robert P. ; Trucco, Massimo ; Ricordi, Camillo ; Allen, Marie ; Noble, Janelle A. ; Erlich, Henry A. ; Eisenbarth, George S. / Sequence analysis of the diabetes-protective human leukocyte antigen-DQB1*0602 allele in unaffected, islet cell antibody-positive first degree relatives and in rare patients with type 1 diabetes. In: Journal of Clinical Endocrinology and Metabolism. 1999 ; Vol. 84, No. 5. pp. 1722-1728.
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abstract = "The human leukocyte antigen (HLA)-DQA1*0102/DQB1*0602/ DRBl*1501 (DR2) haplotype confers strong protection from type 1 diabetes. Growing evidence suggests that such protection may be mostly encoded by the DQB1*0602 allele, and we reported that even first degree relatives with islet cell antibodies (ICA) have an extremely low diabetes risk if they carry DQB1*0602. Recently, novel variants of the DQB1*0602 and *0603 alleles were reported in four patients with type i diabetes originally typed as DQB1*0602 with conventional techniques. One inference from this observation is that DQB1*0602 may confer absolute protection and may never occur in type i diabetes. By this hypothesis, all patients typed as DQB1*0602 positive with conventional techniques should carry one of the above diabetes-permissive variants instead of the protective DQB1*0602. Such variants could also occur in ICA/DQB1*0602-positive relatives, with the implication that their diabetes risk could be significantly higher than previously estimated. We therefore sequenced the DQB1*0602 and DQA1*0102 alleles in all ICA/DQB1*0602-positive relatives (n = 8) previously described and in six rare patients with type i diabetes and DQB1*0602. We found that all relatives and patients carry the known DQB1*0602 and DQA1*0102 sequences, and none of them has the mtDNA A3243G mutation associated with late-onset diabetes in ICA-positive individuals. These findings suggest that diabetes-permissive DQB1*0602/3 variants may be very rare. Thus, although the protective effect associated with DQB1*0602 is extremely powerful, it is not absolute. Nonetheless, the development of diabetes in individuals with DQB1*0602 remains extremely unlikely, even in the presence of ICA, as confirmed by our further evaluation of ICA/DQB1*0602-positive relatives, none of whom has yet developed diabetes.",
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AU - Zeller, Markus

AU - Yu, Liping

AU - Babu, Sunanda

AU - Solimena, Michele

AU - Moraes, Carlos T

AU - Pietropaolo, Massimo

AU - Friday, Robert P.

AU - Trucco, Massimo

AU - Ricordi, Camillo

AU - Allen, Marie

AU - Noble, Janelle A.

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