Abstract
A simple procedure for separation of ribose-5-phosphate, deoxyribose-1-phosphate and ribose-l-phosphate is based on high performance liquid chromatography using reversed phase 4 x 300 mm“pBondapak/NH2” column. The column is equilibrated with 0.13 M borate buffer (pH 7.5) followed by gradient elution of ribose-5-phosphate, deoxyribose-l-phosphate and ribose-l-phosphate using water, 0.05 M borate buffer containing 0.1 M MgCl (pH 9.6) and 0.05 M sodium acetate-acetic acid buffer containing 0.1 M MgCI (pH 5.0) as eluants respectively. Eluates of borate complex “μ Bondapak/NH2” column are brought to pH 9.6 by the addition of 1 N KOH and enzymatically hydrolysed with alkaline phosphatase (EC 3.1.3.1) to.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1265-1277 |
| Number of pages | 13 |
| Journal | Analytical Letters |
| Volume | 16 |
| Issue number | 16 |
| DOIs | |
| State | Published - Jan 1 1983 |
| Externally published | Yes |
Keywords
- 2-Cyanoacetamide
- Deoxyribose-lphosphate
- High performance liquid chromatography
- Ribose-5-phosphate
- Ribose-I-phosphate
- phosphate
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Spectroscopy
- Clinical Biochemistry
- Biochemistry, medical
- Electrochemistry
Fingerprint Dive into the research topics of 'Separation of Ribos E-5-Phosphate, Ribose-L-Phosphate, Deoxyribose-1-Phosphate By High Performance Liquid Chromatography and Spectrophotometric Determination Using 2-Cyanoacetamide'. Together they form a unique fingerprint.
Cite this
Chandrasekaran, B., & Ardalan, B. (1983). Separation of Ribos E-5-Phosphate, Ribose-L-Phosphate, Deoxyribose-1-Phosphate By High Performance Liquid Chromatography and Spectrophotometric Determination Using 2-Cyanoacetamide. Analytical Letters, 16(16), 1265-1277. https://doi.org/10.1080/00032718308065242