Abstract
A simple procedure for separation of ribose-5-phosphate, deoxyribose-1-phosphate and ribose-l-phosphate is based on high performance liquid chromatography using reversed phase 4 x 300 mm“pBondapak/NH2” column. The column is equilibrated with 0.13 M borate buffer (pH 7.5) followed by gradient elution of ribose-5-phosphate, deoxyribose-l-phosphate and ribose-l-phosphate using water, 0.05 M borate buffer containing 0.1 M MgCl (pH 9.6) and 0.05 M sodium acetate-acetic acid buffer containing 0.1 M MgCI (pH 5.0) as eluants respectively. Eluates of borate complex “μ Bondapak/NH2” column are brought to pH 9.6 by the addition of 1 N KOH and enzymatically hydrolysed with alkaline phosphatase (EC 3.1.3.1) to.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1265-1277 |
| Number of pages | 13 |
| Journal | Analytical Letters |
| Volume | 16 |
| Issue number | 16 |
| DOIs | |
| State | Published - Jan 1 1983 |
| Externally published | Yes |
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Keywords
- 2-Cyanoacetamide
- Deoxyribose-lphosphate
- High performance liquid chromatography
- phosphate
- Ribose-5-phosphate
- Ribose-I-phosphate
ASJC Scopus subject areas
- Analytical Chemistry
- Spectroscopy
- Electrochemistry
- Biochemistry, medical
- Clinical Biochemistry
- Biochemistry
Cite this
Separation of Ribos E-5-Phosphate, Ribose-L-Phosphate, Deoxyribose-1-Phosphate By High Performance Liquid Chromatography and Spectrophotometric Determination Using 2-Cyanoacetamide. / Chandrasekaran, Bangaru; Ardalan, Bach.
In: Analytical Letters, Vol. 16, No. 16, 01.01.1983, p. 1265-1277.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Separation of Ribos E-5-Phosphate, Ribose-L-Phosphate, Deoxyribose-1-Phosphate By High Performance Liquid Chromatography and Spectrophotometric Determination Using 2-Cyanoacetamide
AU - Chandrasekaran, Bangaru
AU - Ardalan, Bach
PY - 1983/1/1
Y1 - 1983/1/1
N2 - A simple procedure for separation of ribose-5-phosphate, deoxyribose-1-phosphate and ribose-l-phosphate is based on high performance liquid chromatography using reversed phase 4 x 300 mm“pBondapak/NH2” column. The column is equilibrated with 0.13 M borate buffer (pH 7.5) followed by gradient elution of ribose-5-phosphate, deoxyribose-l-phosphate and ribose-l-phosphate using water, 0.05 M borate buffer containing 0.1 M MgCl (pH 9.6) and 0.05 M sodium acetate-acetic acid buffer containing 0.1 M MgCI (pH 5.0) as eluants respectively. Eluates of borate complex “μ Bondapak/NH2” column are brought to pH 9.6 by the addition of 1 N KOH and enzymatically hydrolysed with alkaline phosphatase (EC 3.1.3.1) to.
AB - A simple procedure for separation of ribose-5-phosphate, deoxyribose-1-phosphate and ribose-l-phosphate is based on high performance liquid chromatography using reversed phase 4 x 300 mm“pBondapak/NH2” column. The column is equilibrated with 0.13 M borate buffer (pH 7.5) followed by gradient elution of ribose-5-phosphate, deoxyribose-l-phosphate and ribose-l-phosphate using water, 0.05 M borate buffer containing 0.1 M MgCl (pH 9.6) and 0.05 M sodium acetate-acetic acid buffer containing 0.1 M MgCI (pH 5.0) as eluants respectively. Eluates of borate complex “μ Bondapak/NH2” column are brought to pH 9.6 by the addition of 1 N KOH and enzymatically hydrolysed with alkaline phosphatase (EC 3.1.3.1) to.
KW - 2-Cyanoacetamide
KW - Deoxyribose-lphosphate
KW - High performance liquid chromatography
KW - phosphate
KW - Ribose-5-phosphate
KW - Ribose-I-phosphate
UR - http://www.scopus.com/inward/record.url?scp=84915203144&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84915203144&partnerID=8YFLogxK
U2 - 10.1080/00032718308065242
DO - 10.1080/00032718308065242
M3 - Article
AN - SCOPUS:84915203144
VL - 16
SP - 1265
EP - 1277
JO - Analytical Letters
JF - Analytical Letters
SN - 0003-2719
IS - 16
ER -