Abstract
A simple procedure for separation of ribose-5-phosphate, deoxyribose-1-phosphate and ribose-l-phosphate is based on high performance liquid chromatography using reversed phase 4 x 300 mm“pBondapak/NH2” column. The column is equilibrated with 0.13 M borate buffer (pH 7.5) followed by gradient elution of ribose-5-phosphate, deoxyribose-l-phosphate and ribose-l-phosphate using water, 0.05 M borate buffer containing 0.1 M MgCl (pH 9.6) and 0.05 M sodium acetate-acetic acid buffer containing 0.1 M MgCI (pH 5.0) as eluants respectively. Eluates of borate complex “μ Bondapak/NH2” column are brought to pH 9.6 by the addition of 1 N KOH and enzymatically hydrolysed with alkaline phosphatase (EC 3.1.3.1) to.
Original language | English (US) |
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Pages (from-to) | 1265-1277 |
Number of pages | 13 |
Journal | Analytical Letters |
Volume | 16 |
Issue number | 16 |
DOIs | |
State | Published - Jan 1 1983 |
Externally published | Yes |
Keywords
- 2-Cyanoacetamide
- Deoxyribose-lphosphate
- High performance liquid chromatography
- Ribose-5-phosphate
- Ribose-I-phosphate
- phosphate
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Spectroscopy
- Clinical Biochemistry
- Biochemistry, medical
- Electrochemistry