Separation of Ribos E-5-Phosphate, Ribose-L-Phosphate, Deoxyribose-1-Phosphate By High Performance Liquid Chromatography and Spectrophotometric Determination Using 2-Cyanoacetamide

Bangaru Chandrasekaran, Bach Ardalan

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

A simple procedure for separation of ribose-5-phosphate, deoxyribose-1-phosphate and ribose-l-phosphate is based on high performance liquid chromatography using reversed phase 4 x 300 mm“pBondapak/NH2” column. The column is equilibrated with 0.13 M borate buffer (pH 7.5) followed by gradient elution of ribose-5-phosphate, deoxyribose-l-phosphate and ribose-l-phosphate using water, 0.05 M borate buffer containing 0.1 M MgCl (pH 9.6) and 0.05 M sodium acetate-acetic acid buffer containing 0.1 M MgCI (pH 5.0) as eluants respectively. Eluates of borate complex “μ Bondapak/NH2” column are brought to pH 9.6 by the addition of 1 N KOH and enzymatically hydrolysed with alkaline phosphatase (EC 3.1.3.1) to.

Original languageEnglish (US)
Pages (from-to)1265-1277
Number of pages13
JournalAnalytical Letters
Volume16
Issue number16
DOIs
StatePublished - Jan 1 1983
Externally publishedYes

Keywords

  • 2-Cyanoacetamide
  • Deoxyribose-lphosphate
  • High performance liquid chromatography
  • Ribose-5-phosphate
  • Ribose-I-phosphate
  • phosphate

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Spectroscopy
  • Clinical Biochemistry
  • Biochemistry, medical
  • Electrochemistry

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