Separation of functionally or highly pure C2 from human plasma with sepharose and a lectin of Euonymus europeus

D. R. Schultz, P. I. Arnold

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

A method is described for isolating both functionally and highly pure C2 from normal human serum or plasma. Functionally pure C2 was obtained by a two-step method of ammonium sulfate (AS) fractionation of plasma or serum and chromatography on AH-Sepharose containing a bound lectin of Euonymus europeus. The functionally pure C2 can be isolated in 2 days, and is used routinely as a reagent for functional hemolytic titrations of C3 and C4 in the Clinical Immunology Laboratory. Highly pure C2 was isolated by the two-step fractionation with AS followed by chromatography on CM-cellulose, aged CNBr-activated Sepharose 4B, and AH-Sepharose-lectin. The major difficulty for isolating highly pure C2 was its separation from Factor B of the alternative complement pathway. The yield of C2 varied from 30 to 40 per cent. Following electrophoresis and staining on polyacrylamide gels, the single band was hemolytically active C2.

Original languageEnglish (US)
Pages (from-to)59-66
Number of pages8
JournalActa Pathologica Microbiologica et Immunologica Scandinavica - Supplementum
Volume92
Issue number284
StatePublished - Jan 1 1984

ASJC Scopus subject areas

  • Medicine(all)

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