Separation of antibody helper and antibody suppressor human T cells by using soybean agglutinin

Y. Reisner, S. Pahwa, J. W. Chiao, N. Sharon, R. L. Evans, R. A. Good

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Soybean agglutinin (SBA) binds specifically to mouse B cells and has been used in the past to separate mouse B and T spleen cells by differential agglutination of the B cells. In the present study it was found that a major T-cell subpopulation of human peripheral blood mononuclear cells is agglutinated by SBA along with the B cells and monocytes. Tests of such cell surface markers as Fc receptors for IgG and IgM, as well as functional assays of antibody production by B cells, revealed that the SBA-agglutinated cell fraction contains the antibody helper T cells whereas the unagglutinated fraction is enriched with antibody suppressor T cells. Similar observations were made in tests of the proliferative response to mumps antigen. A recently prepared monoclonal antibody, anti-Leu 2a, which recognized the same thymus-dependent antigen previously defined by a heterologous anti-human T cell serum (αTH 2), was found to define by indirect immunofluorescence a sub-population of SBA - cells of intermediate staining intensity which was not detectable in the SBA + population.

Original languageEnglish (US)
Pages (from-to)6778-6782
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume77
Issue number11 I
DOIs
StatePublished - Dec 1 1980
Externally publishedYes

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