Separation of antibody helper and antibody suppressor human T cells by using soybean agglutinin

Y. Reisner, Savita G Pahwa, J. W. Chiao, N. Sharon, R. L. Evans, R. A. Good

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Soybean agglutinin (SBA) binds specifically to mouse B cells and has been used in the past to separate mouse B and T spleen cells by differential agglutination of the B cells. In the present study it was found that a major T-cell subpopulation of human peripheral blood mononuclear cells is agglutinated by SBA along with the B cells and monocytes. Tests of such cell surface markers as Fc receptors for IgG and IgM, as well as functional assays of antibody production by B cells, revealed that the SBA-agglutinated cell fraction contains the antibody helper T cells whereas the unagglutinated fraction is enriched with antibody suppressor T cells. Similar observations were made in tests of the proliferative response to mumps antigen. A recently prepared monoclonal antibody, anti-Leu 2a, which recognized the same thymus-dependent antigen previously defined by a heterologous anti-human T cell serum (αTH 2), was found to define by indirect immunofluorescence a sub-population of SBA - cells of intermediate staining intensity which was not detectable in the SBA + population.

Original languageEnglish
Pages (from-to)6778-6782
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume77
Issue number11 I
DOIs
StatePublished - Dec 1 1980
Externally publishedYes

Fingerprint

T-Lymphocytes
B-Lymphocytes
Antibodies
Antigens
Mumps
Agglutination
Indirect Fluorescent Antibody Technique
Helper-Inducer T-Lymphocytes
Thymus Gland
Population
Antibody Formation
Monocytes
Blood Cells
Spleen
Immunoglobulin G
Monoclonal Antibodies
soybean lectin
Staining and Labeling
Serum

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Separation of antibody helper and antibody suppressor human T cells by using soybean agglutinin. / Reisner, Y.; Pahwa, Savita G; Chiao, J. W.; Sharon, N.; Evans, R. L.; Good, R. A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 77, No. 11 I, 01.12.1980, p. 6778-6782.

Research output: Contribution to journalArticle

@article{6a4bb75920a841959a873cd8b658d3de,
title = "Separation of antibody helper and antibody suppressor human T cells by using soybean agglutinin",
abstract = "Soybean agglutinin (SBA) binds specifically to mouse B cells and has been used in the past to separate mouse B and T spleen cells by differential agglutination of the B cells. In the present study it was found that a major T-cell subpopulation of human peripheral blood mononuclear cells is agglutinated by SBA along with the B cells and monocytes. Tests of such cell surface markers as Fc receptors for IgG and IgM, as well as functional assays of antibody production by B cells, revealed that the SBA-agglutinated cell fraction contains the antibody helper T cells whereas the unagglutinated fraction is enriched with antibody suppressor T cells. Similar observations were made in tests of the proliferative response to mumps antigen. A recently prepared monoclonal antibody, anti-Leu 2a, which recognized the same thymus-dependent antigen previously defined by a heterologous anti-human T cell serum (αTH 2), was found to define by indirect immunofluorescence a sub-population of SBA - cells of intermediate staining intensity which was not detectable in the SBA + population.",
author = "Y. Reisner and Pahwa, {Savita G} and Chiao, {J. W.} and N. Sharon and Evans, {R. L.} and Good, {R. A.}",
year = "1980",
month = "12",
day = "1",
doi = "10.1073/pnas.77.11.6778",
language = "English",
volume = "77",
pages = "6778--6782",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "11 I",

}

TY - JOUR

T1 - Separation of antibody helper and antibody suppressor human T cells by using soybean agglutinin

AU - Reisner, Y.

AU - Pahwa, Savita G

AU - Chiao, J. W.

AU - Sharon, N.

AU - Evans, R. L.

AU - Good, R. A.

PY - 1980/12/1

Y1 - 1980/12/1

N2 - Soybean agglutinin (SBA) binds specifically to mouse B cells and has been used in the past to separate mouse B and T spleen cells by differential agglutination of the B cells. In the present study it was found that a major T-cell subpopulation of human peripheral blood mononuclear cells is agglutinated by SBA along with the B cells and monocytes. Tests of such cell surface markers as Fc receptors for IgG and IgM, as well as functional assays of antibody production by B cells, revealed that the SBA-agglutinated cell fraction contains the antibody helper T cells whereas the unagglutinated fraction is enriched with antibody suppressor T cells. Similar observations were made in tests of the proliferative response to mumps antigen. A recently prepared monoclonal antibody, anti-Leu 2a, which recognized the same thymus-dependent antigen previously defined by a heterologous anti-human T cell serum (αTH 2), was found to define by indirect immunofluorescence a sub-population of SBA - cells of intermediate staining intensity which was not detectable in the SBA + population.

AB - Soybean agglutinin (SBA) binds specifically to mouse B cells and has been used in the past to separate mouse B and T spleen cells by differential agglutination of the B cells. In the present study it was found that a major T-cell subpopulation of human peripheral blood mononuclear cells is agglutinated by SBA along with the B cells and monocytes. Tests of such cell surface markers as Fc receptors for IgG and IgM, as well as functional assays of antibody production by B cells, revealed that the SBA-agglutinated cell fraction contains the antibody helper T cells whereas the unagglutinated fraction is enriched with antibody suppressor T cells. Similar observations were made in tests of the proliferative response to mumps antigen. A recently prepared monoclonal antibody, anti-Leu 2a, which recognized the same thymus-dependent antigen previously defined by a heterologous anti-human T cell serum (αTH 2), was found to define by indirect immunofluorescence a sub-population of SBA - cells of intermediate staining intensity which was not detectable in the SBA + population.

UR - http://www.scopus.com/inward/record.url?scp=0019252674&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019252674&partnerID=8YFLogxK

U2 - 10.1073/pnas.77.11.6778

DO - 10.1073/pnas.77.11.6778

M3 - Article

VL - 77

SP - 6778

EP - 6782

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 11 I

ER -