Abstract
Soybean agglutinin (SBA) binds specifically to mouse B cells and has been used in the past to separate mouse B and T spleen cells by differential agglutination of the B cells. In the present study it was found that a major T-cell subpopulation of human peripheral blood mononuclear cells is agglutinated by SBA along with the B cells and monocytes. Tests of such cell surface markers as Fc receptors for IgG and IgM, as well as functional assays of antibody production by B cells, revealed that the SBA-agglutinated cell fraction contains the antibody helper T cells whereas the unagglutinated fraction is enriched with antibody suppressor T cells. Similar observations were made in tests of the proliferative response to mumps antigen. A recently prepared monoclonal antibody, anti-Leu 2a, which recognized the same thymus-dependent antigen previously defined by a heterologous anti-human T cell serum (αTH 2), was found to define by indirect immunofluorescence a sub-population of SBA - cells of intermediate staining intensity which was not detectable in the SBA + population.
| Original language | English |
|---|---|
| Pages (from-to) | 6778-6782 |
| Number of pages | 5 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 77 |
| Issue number | 11 I |
| DOIs | |
| State | Published - Dec 1 1980 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Genetics
- General
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Separation of antibody helper and antibody suppressor human T cells by using soybean agglutinin. / Reisner, Y.; Pahwa, Savita G; Chiao, J. W.; Sharon, N.; Evans, R. L.; Good, R. A.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 77, No. 11 I, 01.12.1980, p. 6778-6782.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Separation of antibody helper and antibody suppressor human T cells by using soybean agglutinin
AU - Reisner, Y.
AU - Pahwa, Savita G
AU - Chiao, J. W.
AU - Sharon, N.
AU - Evans, R. L.
AU - Good, R. A.
PY - 1980/12/1
Y1 - 1980/12/1
N2 - Soybean agglutinin (SBA) binds specifically to mouse B cells and has been used in the past to separate mouse B and T spleen cells by differential agglutination of the B cells. In the present study it was found that a major T-cell subpopulation of human peripheral blood mononuclear cells is agglutinated by SBA along with the B cells and monocytes. Tests of such cell surface markers as Fc receptors for IgG and IgM, as well as functional assays of antibody production by B cells, revealed that the SBA-agglutinated cell fraction contains the antibody helper T cells whereas the unagglutinated fraction is enriched with antibody suppressor T cells. Similar observations were made in tests of the proliferative response to mumps antigen. A recently prepared monoclonal antibody, anti-Leu 2a, which recognized the same thymus-dependent antigen previously defined by a heterologous anti-human T cell serum (αTH 2), was found to define by indirect immunofluorescence a sub-population of SBA - cells of intermediate staining intensity which was not detectable in the SBA + population.
AB - Soybean agglutinin (SBA) binds specifically to mouse B cells and has been used in the past to separate mouse B and T spleen cells by differential agglutination of the B cells. In the present study it was found that a major T-cell subpopulation of human peripheral blood mononuclear cells is agglutinated by SBA along with the B cells and monocytes. Tests of such cell surface markers as Fc receptors for IgG and IgM, as well as functional assays of antibody production by B cells, revealed that the SBA-agglutinated cell fraction contains the antibody helper T cells whereas the unagglutinated fraction is enriched with antibody suppressor T cells. Similar observations were made in tests of the proliferative response to mumps antigen. A recently prepared monoclonal antibody, anti-Leu 2a, which recognized the same thymus-dependent antigen previously defined by a heterologous anti-human T cell serum (αTH 2), was found to define by indirect immunofluorescence a sub-population of SBA - cells of intermediate staining intensity which was not detectable in the SBA + population.
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U2 - 10.1073/pnas.77.11.6778
DO - 10.1073/pnas.77.11.6778
M3 - Article
VL - 77
SP - 6778
EP - 6782
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 11 I
ER -