Separating soil and leaf water 18O isotopic signals in plant stem cellulose

Leonel Sternberg, William T. Anderson, Kanema Morrison

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The oxygen-18 signal of soil and leaf water are both recorded in heterotrophically synthesized plant stem cellulose. Presently, these signals can only be teased apart with modeling and assumptions on the nature of the isotopic enrichment of leaf water. A method by which these two signals are chemically separated and analyzed is tested here. Heterotrophically synthesized cellulose from germinating seeds having a mixture of isotopic signals from the reserve carbohydrate (starch) and that of the water during cellulose synthesis was hydrolyzed and the resulting glucose converted to glucose phenylosazone. The analysis of the 18O/16O ratios of cellulose and of glucose phenylosazone were used to calculate the oxygen isotope ratio of the oxygen attached to the second carbon of the glucose moieties of the cellulose molecule. The calculated δ18O value of this oxygen was highly correlated with that of the water available for cellulose synthesis showing a nearly one-to-one relationship (slope = 1.027) and leading to the conclusion that it completely exchanges with water during heterotrophic cellulose synthesis. Once this method is refined so as to increase precision, it will be possible to derive the δ18O values of soil water available to plants from the oxygen isotope analysis of stem cellulose and its derivative.

Original languageEnglish
Pages (from-to)2561-2566
Number of pages6
JournalGeochimica et Cosmochimica Acta
Volume67
Issue number14
DOIs
StatePublished - Jul 15 2003

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Cellulose
cellulose
stem
Soils
Water
glucose
Oxygen Isotopes
soil
water
Oxygen
oxygen isotope
Glucose
oxygen
oxygen isotope ratio
starch
Starch
Seed
carbohydrate
Ion exchange
Carbon

ASJC Scopus subject areas

  • Geochemistry and Petrology

Cite this

Separating soil and leaf water 18O isotopic signals in plant stem cellulose. / Sternberg, Leonel; Anderson, William T.; Morrison, Kanema.

In: Geochimica et Cosmochimica Acta, Vol. 67, No. 14, 15.07.2003, p. 2561-2566.

Research output: Contribution to journalArticle

Sternberg, Leonel ; Anderson, William T. ; Morrison, Kanema. / Separating soil and leaf water 18O isotopic signals in plant stem cellulose. In: Geochimica et Cosmochimica Acta. 2003 ; Vol. 67, No. 14. pp. 2561-2566.
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