Sensitivity of cultured pancreatic carcinoma cells to Acinetobacter glutaminase-asparaginase

Ming Chi Wu, Grace K. Arimura, John S. Holcenberg, Adel A. Yunis

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Cultured human pancreatic carcinoma cells (MIA PaCa-2) have been shown previously to be very sensitive to E. coli l-asparaginase (EC II). The present studies have demonstrated that another enzyme, Acinetobacter glutaminase-asparaginase (AGA) is much more effective in inhibiting cell growth. At the concentration of 0.0025 U/ml of AGA activity the enzyme totally inhibited cell growth, whereas the EC II with the same concentration did not show any effect. The inhibition of cell growth correlated well with inhibition of protein and glycoprotein synthesis. The addition of l-glutamine at the concentration of 1 m M completely reversed the inhibition of protein synthesis. Similarly, the addition of l-glutamine at the concentration of 3 m M daily on 3 successive days after adding AGA resulted in significant reversal of growth inhibition. The results of this study indicate that the action of AGA on MIA PaCa-2 is, to a great extent, exerted through its l-glutaminase activity.

Original languageEnglish (US)
Pages (from-to)750-754
Number of pages5
JournalIn Vitro
Volume18
Issue number9
DOIs
StatePublished - Sep 1 1982

Keywords

  • asparaginase
  • glutaminase
  • pancreatic carcinoma

ASJC Scopus subject areas

  • Biotechnology
  • Plant Science

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