Abstract
Measurements of carbon monoxide (CO) in breath can be used for the diagnosis of hemolytic disease. A small, semiportable, easy-to-operate CO instrument was developed at Stanford University and tested at 12 Neonatal Research Network Centers of the National Institute of Child Health and Human Development. A syringe pump delivers 7.7 mL of sample per minute through an activated carbon filter to an electrochemical (EC) sensor having a sensitivity of 0.10 ± 0.01 V per 1 μL/L CO in air. The electronically processed sensor signal is displayed on a digital multimeter. For a typical end-tidal CO measurement, corrected for inhaled CO, three 10- to 12-mL breath and room air samples are manually or mechanically collected and analyzed. CO determination in breath samples from 108 healthy, 1 -day-old infants of nonsmoking mothers compared favorably with determinations by gas chromatography (GC), 1.3 ± 0.8 vs 1.2 ± 0.8 (mean ± SD), respectively, with a regression equation of EC = 0.95 GC + 0.13 (r2 = 0.98). The results demonstrate that the EC-CO instrument yields results that are comparable with those obtained by the more difficult to perform GC assay.
Original language | English (US) |
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Pages (from-to) | 1927-1933 |
Number of pages | 7 |
Journal | Clinical chemistry |
Volume | 40 |
Issue number | 10 |
DOIs | |
State | Published - 1994 |
Externally published | Yes |
Keywords
- End-tidal breath analysis
- Gas chromatography compared
- Hyperbilirubinemia
- Jaundice
- Neonates
ASJC Scopus subject areas
- Clinical Biochemistry