Semiautomated analysis of optical coherence tomography crystalline lens images under simulated accommodation

Eon Kim, Klaus Ehrmann, Stephen Uhlhorn, David Borja, Esdras Arrieta-Quintero, Jean Marie Parela

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Abstract

Presbyopia is an age related, gradual loss of accommodation, mainly due to changes in the crystalline lens. As part of research efforts to understand and cure this condition, ex vivo, cross-sectional optical coherence tomography images of crystalline lenses were obtained by using the Ex-Vivo Accommodation Simulator (EVAS II) instrument and analyzed to extract their physical and optical properties. Various filters and edge detectionmethods were applied to isolate the edge contour. An ellipse is fitted to the lens outline to obtain central reference point for transforming the pixel data into the analysis coordinate system. This allows for the fitting of a high order equation to obtain a mathematical description of the edge contour, which obeys constraints of continuity as well as zero to infinite surface slopes from apex to equator. Geometrical parameters of the lenswere determined for the lens images captured at different accommodative states. Various curve fitting functions were developed to mathematically describe the anterior and posterior surfaces of the lens. Their differences were evaluated and their suitability for extracting optical performance of the lens was assessed. The robustness of these algorithms was tested by analyzing the same images repeated times.

Original languageEnglish (US)
Article number056003
JournalJournal of Biomedical Optics
Volume16
Issue number5
DOIs
StatePublished - May 1 2011

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Keywords

  • Accommodation
  • Crystalline lens
  • Image analysis
  • Optical coherence tomography

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

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