Self-Renewal and Differentiation Capacity of Urine-Derived Stem Cells after Urine Preservation for 24 Hours

Ren Lang, Guihua Liu, Yingai Shi, Shantaram Bharadwaj, Xiaoyan Leng, Xiaobo Zhou, Hong Liu, Anthony Atala, Yuanyuan Zhang

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Despite successful approaches to preserve organs, tissues, and isolated cells, the maintenance of stem cell viability and function in body fluids during storage for cell distribution and transportation remains unexplored. The aim of this study was to characterize urine-derived stem cells (USCs) after optimal preservation of urine specimens for up to 24 hours. A total of 415 urine specimens were collected from 12 healthy men (age range 20-54 years old). About 6×104 cells shed off from the urinary tract system in 24 hours. At least 100 USC clones were obtained from the stored urine specimens after 24 hours and maintained similar biological features to fresh USCs. The stored USCs had a "rice grain" shape in primary culture, and expressed mesenchymal stem cell surface markers, high telomerase activity, and normal karyotypes. Importantly, the preserved cells retained bipotent differentiation capacity. Differentiated USCs expressed myogenic specific proteins and contractile function when exposed to myogenic differentiation medium, and they expressed urothelial cell-specific markers and barrier function when exposed to urothelial differentiation medium. These data demonstrated that up to 75% of fresh USCs can be safely persevered in urine for 24 hours and that these cells stored in urine retain their original stem cell properties, indicating that preserved USCs could be available for potential use in cell-based therapy or clinical diagnosis.

Original languageEnglish
Article numbere53980
JournalPLoS One
Volume8
Issue number1
DOIs
StatePublished - Jan 18 2013
Externally publishedYes

Fingerprint

Stem cells
stem cells
urine
Stem Cells
Urine
cells
Contractile Proteins
Telomerase
Body fluids
telomerase
Cell culture
body fluids
urinary tract
Body Fluids
Cell- and Tissue-Based Therapy
Urinary Tract
Mesenchymal Stromal Cells
Karyotype
karyotyping
cell viability

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Self-Renewal and Differentiation Capacity of Urine-Derived Stem Cells after Urine Preservation for 24 Hours. / Lang, Ren; Liu, Guihua; Shi, Yingai; Bharadwaj, Shantaram; Leng, Xiaoyan; Zhou, Xiaobo; Liu, Hong; Atala, Anthony; Zhang, Yuanyuan.

In: PLoS One, Vol. 8, No. 1, e53980, 18.01.2013.

Research output: Contribution to journalArticle

Lang, R, Liu, G, Shi, Y, Bharadwaj, S, Leng, X, Zhou, X, Liu, H, Atala, A & Zhang, Y 2013, 'Self-Renewal and Differentiation Capacity of Urine-Derived Stem Cells after Urine Preservation for 24 Hours', PLoS One, vol. 8, no. 1, e53980. https://doi.org/10.1371/journal.pone.0053980
Lang, Ren ; Liu, Guihua ; Shi, Yingai ; Bharadwaj, Shantaram ; Leng, Xiaoyan ; Zhou, Xiaobo ; Liu, Hong ; Atala, Anthony ; Zhang, Yuanyuan. / Self-Renewal and Differentiation Capacity of Urine-Derived Stem Cells after Urine Preservation for 24 Hours. In: PLoS One. 2013 ; Vol. 8, No. 1.
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