Selective toxicity of rhodamine 123 in carcinoma cells in vitro

Theodore Lampidis, S. D. Bernal, I. C. Summerhayes, Be Chen Lan Be Chen

Research output: Contribution to journalArticle

226 Citations (Scopus)

Abstract

The study of mitochondria in situ has recently been facilitated through the use of rhodamine 123, a mitochondrial-specific fluorescent dye. It has been found to be nontoxic when applied for short periods to a variety of cell types and has thus become an invaluable tool for examining mitochondrial morphology and function in the intact living cell. In this report, however, we demonstrate that with continuous exposure, rhodamine 123 selectively kills carcinoma as compared to normal epithelial cells grown in vitro. At doses of rhodamine 123 which were toxic to carcinoma cells, the conversion of mitochondrial-specific to cytoplasmic-nonspecific localization of the drug was observed prior to cell death. At 10 μg/ml, >50% cell death occurred within 7 days in all nine of the carcinoma cell types and lines of different origin studied, while six of six normal epithelial cell types and lines remained unaffected. Cotreating carcinoma cells with 2-deoxyglucose and rhodamine 123 enhanced the inhibition of growth by rhodamine 123 alone in clonogenic survival assays. The observation of the selective toxicity of rhodamine 123 appears to be unique in view of the absence of selective toxicity reported in vitro for the various antitumor agents currently in clinical use. Preliminary results with rhodamine 123 in animal tumor systems indicate antitumor activity for carcinomas.

Original languageEnglish
Pages (from-to)716-720
Number of pages5
JournalCancer Research
Volume43
Issue number2
StatePublished - Jan 1 1983
Externally publishedYes

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Rhodamine 123
Carcinoma
Cell Death
Epithelial Cells
Cell Line
Poisons
Deoxyglucose
In Vitro Techniques
Fluorescent Dyes
Antineoplastic Agents
Mitochondria
Observation
Growth
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Lampidis, T., Bernal, S. D., Summerhayes, I. C., & Lan Be Chen, B. C. (1983). Selective toxicity of rhodamine 123 in carcinoma cells in vitro. Cancer Research, 43(2), 716-720.

Selective toxicity of rhodamine 123 in carcinoma cells in vitro. / Lampidis, Theodore; Bernal, S. D.; Summerhayes, I. C.; Lan Be Chen, Be Chen.

In: Cancer Research, Vol. 43, No. 2, 01.01.1983, p. 716-720.

Research output: Contribution to journalArticle

Lampidis, T, Bernal, SD, Summerhayes, IC & Lan Be Chen, BC 1983, 'Selective toxicity of rhodamine 123 in carcinoma cells in vitro', Cancer Research, vol. 43, no. 2, pp. 716-720.
Lampidis T, Bernal SD, Summerhayes IC, Lan Be Chen BC. Selective toxicity of rhodamine 123 in carcinoma cells in vitro. Cancer Research. 1983 Jan 1;43(2):716-720.
Lampidis, Theodore ; Bernal, S. D. ; Summerhayes, I. C. ; Lan Be Chen, Be Chen. / Selective toxicity of rhodamine 123 in carcinoma cells in vitro. In: Cancer Research. 1983 ; Vol. 43, No. 2. pp. 716-720.
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