Selective neuronal, dendritic, and postsynaptic localization of viral antigen in measles-infected mice

C. Van Pottelsberghe, Kottil W Rammohan, H. F. McFarland, M. Dubois-Dalcq

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The pathology of acute disease produced by intracerebral inoculation of hamster neurotropic strain of measles virus was studied in adult BALB/c mice using immunolabeling techniques at a light and electron microscopic level. Brains of animals with acute disease showed an abundance of viral antigen but no inflammatory cells, giant cells, or inclusions. Infection was limited to neurons which were rarely necrotic, indicating that the process was not cytolytic. Mapping of infected neurons identified a consistent predilection to the rhinencephalon, other components of the limbic system, and the striatum. Ultrastructural examination revealed similar findings in all of the involved areas. No evidence of viral assembly at the cell membrane was found. Viral antigen was identified in neuronal perikaryon with somatofugal spread into dendritic and synaptic sites. Unlabeled smooth nucleocapsid and labeled tubular structures were detected both in the cytoplasm and nucleus of neurons. Dendritic labeling when present was occasionally associated with the neurotubules. The most remarkable and frequent finding was identification of viral antigen in postsynaptic endings. This consisted of clumps of viral antigen and occasional staining of the postsynaptic density. This localization of viral antigen may create dysfunction of synaptic transmission, and in the absence of overt pathology, may account for clinical disease.

Original languageEnglish
Pages (from-to)99-108
Number of pages10
JournalLaboratory Investigation
Volume40
Issue number1
StatePublished - Jan 1 1979
Externally publishedYes

Fingerprint

Viral Antigens
Measles
Acute Disease
Neurons
Pathology
Post-Synaptic Density
Virus Assembly
Nucleocapsid
Limbic System
Measles virus
Giant Cells
Synaptic Transmission
Cricetinae
Cytoplasm
Immunohistochemistry
Cell Membrane
Electrons
Staining and Labeling
Light
Brain

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Selective neuronal, dendritic, and postsynaptic localization of viral antigen in measles-infected mice. / Van Pottelsberghe, C.; Rammohan, Kottil W; McFarland, H. F.; Dubois-Dalcq, M.

In: Laboratory Investigation, Vol. 40, No. 1, 01.01.1979, p. 99-108.

Research output: Contribution to journalArticle

Van Pottelsberghe, C. ; Rammohan, Kottil W ; McFarland, H. F. ; Dubois-Dalcq, M. / Selective neuronal, dendritic, and postsynaptic localization of viral antigen in measles-infected mice. In: Laboratory Investigation. 1979 ; Vol. 40, No. 1. pp. 99-108.
@article{81e845d9ed4e4ab292893db53a140ca5,
title = "Selective neuronal, dendritic, and postsynaptic localization of viral antigen in measles-infected mice",
abstract = "The pathology of acute disease produced by intracerebral inoculation of hamster neurotropic strain of measles virus was studied in adult BALB/c mice using immunolabeling techniques at a light and electron microscopic level. Brains of animals with acute disease showed an abundance of viral antigen but no inflammatory cells, giant cells, or inclusions. Infection was limited to neurons which were rarely necrotic, indicating that the process was not cytolytic. Mapping of infected neurons identified a consistent predilection to the rhinencephalon, other components of the limbic system, and the striatum. Ultrastructural examination revealed similar findings in all of the involved areas. No evidence of viral assembly at the cell membrane was found. Viral antigen was identified in neuronal perikaryon with somatofugal spread into dendritic and synaptic sites. Unlabeled smooth nucleocapsid and labeled tubular structures were detected both in the cytoplasm and nucleus of neurons. Dendritic labeling when present was occasionally associated with the neurotubules. The most remarkable and frequent finding was identification of viral antigen in postsynaptic endings. This consisted of clumps of viral antigen and occasional staining of the postsynaptic density. This localization of viral antigen may create dysfunction of synaptic transmission, and in the absence of overt pathology, may account for clinical disease.",
author = "{Van Pottelsberghe}, C. and Rammohan, {Kottil W} and McFarland, {H. F.} and M. Dubois-Dalcq",
year = "1979",
month = "1",
day = "1",
language = "English",
volume = "40",
pages = "99--108",
journal = "Laboratory Investigation",
issn = "0023-6837",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - Selective neuronal, dendritic, and postsynaptic localization of viral antigen in measles-infected mice

AU - Van Pottelsberghe, C.

AU - Rammohan, Kottil W

AU - McFarland, H. F.

AU - Dubois-Dalcq, M.

PY - 1979/1/1

Y1 - 1979/1/1

N2 - The pathology of acute disease produced by intracerebral inoculation of hamster neurotropic strain of measles virus was studied in adult BALB/c mice using immunolabeling techniques at a light and electron microscopic level. Brains of animals with acute disease showed an abundance of viral antigen but no inflammatory cells, giant cells, or inclusions. Infection was limited to neurons which were rarely necrotic, indicating that the process was not cytolytic. Mapping of infected neurons identified a consistent predilection to the rhinencephalon, other components of the limbic system, and the striatum. Ultrastructural examination revealed similar findings in all of the involved areas. No evidence of viral assembly at the cell membrane was found. Viral antigen was identified in neuronal perikaryon with somatofugal spread into dendritic and synaptic sites. Unlabeled smooth nucleocapsid and labeled tubular structures were detected both in the cytoplasm and nucleus of neurons. Dendritic labeling when present was occasionally associated with the neurotubules. The most remarkable and frequent finding was identification of viral antigen in postsynaptic endings. This consisted of clumps of viral antigen and occasional staining of the postsynaptic density. This localization of viral antigen may create dysfunction of synaptic transmission, and in the absence of overt pathology, may account for clinical disease.

AB - The pathology of acute disease produced by intracerebral inoculation of hamster neurotropic strain of measles virus was studied in adult BALB/c mice using immunolabeling techniques at a light and electron microscopic level. Brains of animals with acute disease showed an abundance of viral antigen but no inflammatory cells, giant cells, or inclusions. Infection was limited to neurons which were rarely necrotic, indicating that the process was not cytolytic. Mapping of infected neurons identified a consistent predilection to the rhinencephalon, other components of the limbic system, and the striatum. Ultrastructural examination revealed similar findings in all of the involved areas. No evidence of viral assembly at the cell membrane was found. Viral antigen was identified in neuronal perikaryon with somatofugal spread into dendritic and synaptic sites. Unlabeled smooth nucleocapsid and labeled tubular structures were detected both in the cytoplasm and nucleus of neurons. Dendritic labeling when present was occasionally associated with the neurotubules. The most remarkable and frequent finding was identification of viral antigen in postsynaptic endings. This consisted of clumps of viral antigen and occasional staining of the postsynaptic density. This localization of viral antigen may create dysfunction of synaptic transmission, and in the absence of overt pathology, may account for clinical disease.

UR - http://www.scopus.com/inward/record.url?scp=0018351745&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018351745&partnerID=8YFLogxK

M3 - Article

C2 - 368437

AN - SCOPUS:0018351745

VL - 40

SP - 99

EP - 108

JO - Laboratory Investigation

JF - Laboratory Investigation

SN - 0023-6837

IS - 1

ER -