Selection of genetic variants of simian immunodeficiency virus in persistently infected rhesus monkeys

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180 Citations (Scopus)

Abstract

Genetic and antigenic variation may be one means by which lentiviruses that cause AIDS avoid elimination by host immune responses. Genetic variation in the envelope gene (env) was studied by comparing the nucleotide sequences of 27 clones obtained from two rhesus monkeys infected with molecularly cloned simian immunodeficiency virus. All 27 clones differed from each other and differed from the input clone in the gp120 (SU) portion of the envelope gene. Nucleotide substitutions were shown to accumulate with time at an average rate of 8.5 per 1,000 per year in SU. Surprisingly, the majority of nucleotide substitutions (81%) resulted in amino acid changes. Variation in SU was not random but occurred predominantly in five discrete regions. Within these variable regions, a remarkable 98% of the nucleotide substitutions changed the amino acid. These results demonstrate that extensive sequence variability accumulates in vivo after infection with molecularly cloned virus and that selection occurs in vivo for changes in distinct variable regions of env.

Original languageEnglish (US)
Pages (from-to)1843-1854
Number of pages12
JournalJournal of Virology
Volume65
Issue number4
StatePublished - 1991
Externally publishedYes

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Simian immunodeficiency virus
Simian Immunodeficiency Virus
Macaca mulatta
Nucleotides
Clone Cells
nucleotides
clones
env Genes
Antigenic Variation
antigenic variation
Lentivirus
genetic variation
amino acid substitution
Amino Acid Substitution
Acquired Immunodeficiency Syndrome
genes
immune response
Viruses
nucleotide sequences
Amino Acids

ASJC Scopus subject areas

  • Immunology

Cite this

Selection of genetic variants of simian immunodeficiency virus in persistently infected rhesus monkeys. / Burns, D. P W; Desrosiers, Ronald Charles.

In: Journal of Virology, Vol. 65, No. 4, 1991, p. 1843-1854.

Research output: Contribution to journalArticle

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