Selection of donors significantly improves pig islet isolation yield

C. Socci, C. Ricordi, A. M. Davalli, C. Staudacher, P. Baro, A. Vertova, M. Freschi, F. Gavazzi, S. Braghi, G. Pozza, V. Di Carlo

Research output: Contribution to journalArticle

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Abstract

The purpose of the present study was to test the influence of donor age onislet isolation yield in swines. Large White pigs of 10 months (group 1, n = 10) and 2-3 years (group 2, n = 5) were used. A modification of the automated method for human islet isolation was used for pig islet separation. After ductal injection of a collagenase solution (Seromed, type C- 1000) the islets were separated through a continuous digestion process. In group 1 it was possible to obtain an average of 636,100 islets (volume = 476.1 mm3, insulin content = 101.4 Units). In group 2 the average total islet number was 799,800 representing a volume of 1190.6 mm3 (insulin content 210 Units). Total islet number was not significantly different in the two groups, however when islet volume and total insulin content were considered, older animals allowed a significantly higher yield (P<0.01 for volume and P<0.05 for insulin content). These findings indicate that it is possible to separate large number of pig islets from both young (10 months) and older (2-3 years) animals and that selection of donor pigs will improve islet isolation yield. These results may be of assistance in the definition of requirements for more effective islet isolation in swines.

Original languageEnglish (US)
Pages (from-to)32-34
Number of pages3
JournalHormone and Metabolic Research
Volume25
Issue numberSUPPL.
StatePublished - Dec 1 1990
Externally publishedYes

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

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    Socci, C., Ricordi, C., Davalli, A. M., Staudacher, C., Baro, P., Vertova, A., Freschi, M., Gavazzi, F., Braghi, S., Pozza, G., & Di Carlo, V. (1990). Selection of donors significantly improves pig islet isolation yield. Hormone and Metabolic Research, 25(SUPPL.), 32-34.