Seizure-induced protein tyrosine phosphorylation in rat brain regions

Richard S Jope, G. V W Johnson, M. S. Baird

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Phosphorylation of protein tyrosines is an important modulatory process for cell signaling and other cellular functions. Rat brain regions were examined for altered protein phosphotyrosines, using Western blot analysis and microwave irradiation to limit postmortem alterations, after administration of two convulsants: lithium plus pilocarpine or kainic acid (KA). Most phosphotyrosine proteins were unaltered by these treatments, but there was a large, specific increase in the tyrosine phosphorylation of a 40-Kd protein. This increase was evident in all three regions examined: cerebral cortex, hippocampus, and striatum; it occurred abruptly with onset of generalized status epilepticus (SE) and remained elevated for at least 90 min. Most of the tyrosine phosphorylated 40-Kd protein was in the cytosolic fraction. These results demonstrate a large, specific effect of chemically induced seizures on a single phosphotyrosine protein in rat brain.

Original languageEnglish
Pages (from-to)755-760
Number of pages6
JournalEpilepsia
Volume32
Issue number6
StatePublished - Dec 1 1991
Externally publishedYes

Fingerprint

Tyrosine
Seizures
Phosphorylation
Phosphotyrosine
Brain
Proteins
Convulsants
Pilocarpine
Status Epilepticus
Kainic Acid
Microwaves
Lithium
Cerebral Cortex
Hippocampus
Western Blotting

Keywords

  • Epilepsy
  • Phosphotyrosine
  • Protein phosphorylation
  • Status epilepticus

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Jope, R. S., Johnson, G. V. W., & Baird, M. S. (1991). Seizure-induced protein tyrosine phosphorylation in rat brain regions. Epilepsia, 32(6), 755-760.

Seizure-induced protein tyrosine phosphorylation in rat brain regions. / Jope, Richard S; Johnson, G. V W; Baird, M. S.

In: Epilepsia, Vol. 32, No. 6, 01.12.1991, p. 755-760.

Research output: Contribution to journalArticle

Jope, RS, Johnson, GVW & Baird, MS 1991, 'Seizure-induced protein tyrosine phosphorylation in rat brain regions', Epilepsia, vol. 32, no. 6, pp. 755-760.
Jope, Richard S ; Johnson, G. V W ; Baird, M. S. / Seizure-induced protein tyrosine phosphorylation in rat brain regions. In: Epilepsia. 1991 ; Vol. 32, No. 6. pp. 755-760.
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