Segmental exchange between MHC class I genes in a higher primate

recombination in the gorilla between the ancestor of a human non-functional gene and an A locus gene

David Watkins, Zheng W. Chen, Theodore L. Garber, Austin L. Hughes, Norman L. Letvin

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Classical human major histocompatibility complex (MHC) class I molecules are the products of highly diverse gene loci. It has been suggested that segmental exchange may play a role in the generation of diversity at the antigen recognition site of MHC class I molecules. Here we present the cloning, sequencing and expression of two gorilla A locus cDNAs. One of these cDNAs shows remarkable similarity to the non-functional HLA-AR locus gene (5.4-LBF) only in exon 2. The remainder of the cDNA, however, is most closely related to other classical higher primate A locus genes. This suggests that a segmental exchange may have occurred between the ancestor of the non-functional HLA-AR gene and a classical gorilla A locus gene. Furthermore, the recombination event resulting in Gogo-A3 has affected its antigen recognition site. These data, therefore, demonstrate that segmental exchange can generate diversity at the antigen recognition sites of primate MHC class I molecules and suggest that non-functional genes can contribute to the generation of diversity of classical MHC class I genes.

Original languageEnglish
Pages (from-to)185-191
Number of pages7
JournalImmunogenetics
Volume34
Issue number3
DOIs
StatePublished - Sep 1 1991
Externally publishedYes

Fingerprint

Gorilla gorilla
MHC Class I Genes
Major Histocompatibility Complex
Primates
Genetic Recombination
Genes
Complementary DNA
Antigens
Organism Cloning
Exons

ASJC Scopus subject areas

  • Immunology
  • Genetics

Cite this

Segmental exchange between MHC class I genes in a higher primate : recombination in the gorilla between the ancestor of a human non-functional gene and an A locus gene. / Watkins, David; Chen, Zheng W.; Garber, Theodore L.; Hughes, Austin L.; Letvin, Norman L.

In: Immunogenetics, Vol. 34, No. 3, 01.09.1991, p. 185-191.

Research output: Contribution to journalArticle

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