Secretion of acetylcholinesterase: relation to acetylcholine receptor metabolism.

Richard L Rotundo, D. M. Fambrough

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Acetylcholinesterase (AChE) and acetylcholine receptors (AChR) are muscle-specific glycoproteins present (AChR) are muscle-specific glycoproteins present in cultured chick embryo muscle cells. The first is found as both a secreted and a membrane-bound enzyme whereas the ACh receptor is strictly an integral membrane protein. We have studied the transport and externalization of these two proteins in the same cells using several compounds known to affect secretory processes: colchicine, tunicamycin and the ionophores X-537A, Nigericin and Monensin. Under all experimental conditions, any change in the rate of AChE secretion was accompanied by an identical change in the rate of ACh receptor incorporation into the plasma membrane. These studies were designed to test directly the hypothesis that secreted and integral membrane proteins are transported together to the plasma membrane. Our results are consistent with a single transport pathway in muscle cells for the externalization of membrane and secreted proteins.

Original languageEnglish
Pages (from-to)595-602
Number of pages8
JournalCell
Volume22
Issue number2 Pt 2
StatePublished - Nov 1 1980
Externally publishedYes

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Cholinergic Receptors
Acetylcholinesterase
Metabolism
Muscle
Membrane Proteins
Cell Membrane
Cell membranes
Muscle Cells
Glycoproteins
Lasalocid
Cells
Nigericin
Membranes
Tunicamycin
Monensin
Muscles
Secretory Pathway
Ionophores
Colchicine
Chick Embryo

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Secretion of acetylcholinesterase : relation to acetylcholine receptor metabolism. / Rotundo, Richard L; Fambrough, D. M.

In: Cell, Vol. 22, No. 2 Pt 2, 01.11.1980, p. 595-602.

Research output: Contribution to journalArticle

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