Secreted phosphatase activities in trypanosomatid parasites of plants modulated by platelet-activating factor

P. M.L. Dutra, F. A. Dias, M. A.A. Santos, C. O. Rodrigues, A. Romeiro, M. Attias, W. De Souza, A. H.C.S. Lopes, J. R. Meyer-Fernandes

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22 Scopus citations

Abstract

The secreted phosphatase activities of two trypanosomatid parasites were characterized and compared with supernatants of living cells. The plant parasite Phytomonas françai and the phytophagous hemipteran parasite Herpetomonas sp. hydrolyzed p-nitrophenylphosphate at a rate of 15.54 and 6.51 nmol Pi/mg of protein per min, respectively. Sodium orthovanadate (NaVO3) and sodium fluoride (NaF) decreased the phosphatase activities. The phosphatase activity of P. françai was drastically diminished (73% inhibition) in the presence of sodium tartrate, whereas the phosphatase activity of Herpetomonas sp. was inhibited by 23%. Cytochemical analysis showed the localization of these enzymes on the external surface and in the flagellar pocket of the two trypanosomatids. Sodium tartrate inhibited this reaction, confirming the biochemical data. Platelet-activating factor modulated the phosphatase activities, inhibiting P. françai activity and stimulating Herpetomonas sp. phosphatase activity.

Original languageEnglish (US)
Pages (from-to)408-414
Number of pages7
JournalPhytopathology
Volume91
Issue number4
DOIs
StatePublished - Jan 1 2001
Externally publishedYes

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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    Dutra, P. M. L., Dias, F. A., Santos, M. A. A., Rodrigues, C. O., Romeiro, A., Attias, M., De Souza, W., Lopes, A. H. C. S., & Meyer-Fernandes, J. R. (2001). Secreted phosphatase activities in trypanosomatid parasites of plants modulated by platelet-activating factor. Phytopathology, 91(4), 408-414. https://doi.org/10.1094/PHYTO.2001.91.4.408