Sec17p and HOPS, in distinct SNARE complexes, mediate SNARE complex disruption or assembly for fusion

Kevin M. Collins, Naomi L. Thorngren, Rutilio A. Fratti, William T. Wickner

Research output: Contribution to journalArticlepeer-review

83 Scopus citations

Abstract

SNARE functions during membrane docking and fusion are regulated by Sec1/Munc18 (SM) chaperones and Rab/Ypt GTPase effectors. These functions for yeast vacuole fusion are combined in the six-subunit HOPS complex. HOPS facilitates Ypt7p nucleotide exchange, is a Ypt7p effector, and contains an SM protein. We have dissected the associations and requirements for HOPS, Ypt7p, and Sec17/18p during SNARE complex assembly. Vacuole SNARE complexes bind either Sec17p or the HOPS complex, but not both. Sec17p and its co-chaperone Sec18p disassemble SNARE complexes. Ypt7p regulates the re-assembly of unpaired SNAREs with each other and with HOPS, forming HOPS · SNARE complexes prior to fusion. After HOPS · SNARE assembly, lipid rearrangements are still required for vacuole content mixing. Thus, Sec17p and HOPS have mutually exclusive interactions with vacuole SNAREs to mediate disruption of SNARE complexes or their assembly for docking and fusion. Sec17p may displace HOPS from SNAREs to permit subsequent rounds of fusion.

Original languageEnglish (US)
Pages (from-to)1775-1786
Number of pages12
JournalEMBO Journal
Volume24
Issue number10
DOIs
StatePublished - May 18 2005
Externally publishedYes

Keywords

  • Fusion
  • HOPS
  • Membrane
  • Rab
  • SNARE

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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