Schwann cell but not olfactory ensheathing glia transplants improve hindlimb locomotor performance in the moderately contused adult rat thoracic spinal cord

Toshihiro Takami, Martin Oudega, Margaret L. Bates, Patrick Wood, Naomi Kleitman, Mary B Bunge

Research output: Contribution to journalArticle

397 Citations (Scopus)

Abstract

Cultured adult rat Schwann cells (SCs) or olfactory ensheathing glia (OEG), or both, were transplanted in the adult Fischer rat thoracic (T9) spinal cord 1 week after a moderate contusion (10 gm, 12.5 mm, NYU impactor). Rats received either a total of 2 x 106 cells suspended in culture medium or culture medium only (controls). At 12 weeks after injury, all grafted animals exhibited diminished cavitation. Although in medium-injected rats 33% of spinal tissue within a 5-mm-long segment of cord centered at the injury site was spared, significantly more tissue was spared in SC (51%), OEG (43%), and SC/OEG (44%) grafted animals. All three types of glial grafts were filled with axons, primarily of spinal origin. SC grafts contained more myelinated axons than SC/OEG and OEG grafts. Both types of SC-containing grafts expressed more intense staining for glial fibrillary acidic protein and chondroitin sulfate proteoglycan compared with OEG-only grafts. Retrograde tracing demonstrated that the number of propriospinal and brainstem axons reaching 5-6 mm beyond the grafted area was significantly higher with SC and SC/OEG grafts but not with OEG-only grafts compared with controls. Corticospinal fibers terminated closer to the lesion epicenter in all grafted animals than in controls. With SC-only grafts, a modest but statistically significant improvement in hindlimb locomotor performance was detected at 8-11 weeks after injury. Thus, in addition to this functional improvement, our results show that an SC graft is more effective in promoting axonal sparing/regeneration than an SC/OEG or OEG graft in the moderately contused adult rat thoracic spinal cord.

Original languageEnglish
Pages (from-to)6670-6681
Number of pages12
JournalJournal of Neuroscience
Volume22
Issue number15
StatePublished - Aug 1 2002

Fingerprint

Contusions
Schwann Cells
Hindlimb
Neuroglia
Spinal Cord
Thorax
Transplants
Axons
Culture Media
Wounds and Injuries
Chondroitin Sulfate Proteoglycans
Glial Fibrillary Acidic Protein
Inbred F344 Rats
Brain Stem
Regeneration
Staining and Labeling

Keywords

  • Axonal regeneration
  • Axonal sparing
  • Brainstem axons
  • Contusion injury
  • Corticospinal tract
  • Neuroprotection
  • Propriospinal axons
  • Spinal cord injury
  • Transplantation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Schwann cell but not olfactory ensheathing glia transplants improve hindlimb locomotor performance in the moderately contused adult rat thoracic spinal cord. / Takami, Toshihiro; Oudega, Martin; Bates, Margaret L.; Wood, Patrick; Kleitman, Naomi; Bunge, Mary B.

In: Journal of Neuroscience, Vol. 22, No. 15, 01.08.2002, p. 6670-6681.

Research output: Contribution to journalArticle

Takami, Toshihiro ; Oudega, Martin ; Bates, Margaret L. ; Wood, Patrick ; Kleitman, Naomi ; Bunge, Mary B. / Schwann cell but not olfactory ensheathing glia transplants improve hindlimb locomotor performance in the moderately contused adult rat thoracic spinal cord. In: Journal of Neuroscience. 2002 ; Vol. 22, No. 15. pp. 6670-6681.
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AU - Oudega, Martin

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AU - Wood, Patrick

AU - Kleitman, Naomi

AU - Bunge, Mary B

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