Schisis contributes to necrosis in experimental HSV-1 retinitis

Scott W. Cousins, Norman H. Altman, Sally S. Atherton

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Injection of the KOS strain of herpes simplex virus type 1 into the anterior chamber of one eye of a BALB c mouse produces bilateral retinal inflammation which progresses in the contralateral eye to total retinal necrosis by day 10 post-inoculation. Although both immune and direct viral cytopathic effects have been proposed as causes of the initial infiltration, the precise mechanism(s) of the contralateral retinal necrosis remains uncertain. In this study, histopathological examination of the uninoculated eyes of HSV-1 injected BALB c mice revealed the presence of vitreous traction against the internal limiting membrane of the retina which was associated with separation of the nerve fiber layer from the underlying ganglion cell layer. This separation resulted in avulsion of Mueller cell footplates and retinal vessels, hemorrhage into the schisis cavity and disruption of the underlying retinal architecture. The schisis began focally on day 8 or 9 post-inoculation, and the entire retina of most animals was involved by day 10 or 11. These results suggest that retinal schisis involving separation of the nerve fiber layer from the ganglion cell layer contributes to necrosis of the retina in the uninoculated contralateral eye.

Original languageEnglish
Pages (from-to)745-760
Number of pages16
JournalExperimental Eye Research
Volume48
Issue number6
DOIs
StatePublished - Jan 1 1989
Externally publishedYes

Fingerprint

Retinitis
Human Herpesvirus 1
Necrosis
Retina
Nerve Fibers
Ganglia
Viral Cytopathogenic Effect
Retinal Hemorrhage
Retinal Vessels
Traction
Anterior Chamber
Inflammation
Injections
Membranes

Keywords

  • herpes simplex virus type 1
  • retinitis
  • schisis
  • vitreoretinal traction

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Schisis contributes to necrosis in experimental HSV-1 retinitis. / Cousins, Scott W.; Altman, Norman H.; Atherton, Sally S.

In: Experimental Eye Research, Vol. 48, No. 6, 01.01.1989, p. 745-760.

Research output: Contribution to journalArticle

Cousins, Scott W. ; Altman, Norman H. ; Atherton, Sally S. / Schisis contributes to necrosis in experimental HSV-1 retinitis. In: Experimental Eye Research. 1989 ; Vol. 48, No. 6. pp. 745-760.
@article{b45dea24fdbe481585a193a24c3069f7,
title = "Schisis contributes to necrosis in experimental HSV-1 retinitis",
abstract = "Injection of the KOS strain of herpes simplex virus type 1 into the anterior chamber of one eye of a BALB c mouse produces bilateral retinal inflammation which progresses in the contralateral eye to total retinal necrosis by day 10 post-inoculation. Although both immune and direct viral cytopathic effects have been proposed as causes of the initial infiltration, the precise mechanism(s) of the contralateral retinal necrosis remains uncertain. In this study, histopathological examination of the uninoculated eyes of HSV-1 injected BALB c mice revealed the presence of vitreous traction against the internal limiting membrane of the retina which was associated with separation of the nerve fiber layer from the underlying ganglion cell layer. This separation resulted in avulsion of Mueller cell footplates and retinal vessels, hemorrhage into the schisis cavity and disruption of the underlying retinal architecture. The schisis began focally on day 8 or 9 post-inoculation, and the entire retina of most animals was involved by day 10 or 11. These results suggest that retinal schisis involving separation of the nerve fiber layer from the ganglion cell layer contributes to necrosis of the retina in the uninoculated contralateral eye.",
keywords = "herpes simplex virus type 1, retinitis, schisis, vitreoretinal traction",
author = "Cousins, {Scott W.} and Altman, {Norman H.} and Atherton, {Sally S.}",
year = "1989",
month = "1",
day = "1",
doi = "10.1016/0014-4835(89)90061-4",
language = "English",
volume = "48",
pages = "745--760",
journal = "Experimental Eye Research",
issn = "0014-4835",
publisher = "Academic Press Inc.",
number = "6",

}

TY - JOUR

T1 - Schisis contributes to necrosis in experimental HSV-1 retinitis

AU - Cousins, Scott W.

AU - Altman, Norman H.

AU - Atherton, Sally S.

PY - 1989/1/1

Y1 - 1989/1/1

N2 - Injection of the KOS strain of herpes simplex virus type 1 into the anterior chamber of one eye of a BALB c mouse produces bilateral retinal inflammation which progresses in the contralateral eye to total retinal necrosis by day 10 post-inoculation. Although both immune and direct viral cytopathic effects have been proposed as causes of the initial infiltration, the precise mechanism(s) of the contralateral retinal necrosis remains uncertain. In this study, histopathological examination of the uninoculated eyes of HSV-1 injected BALB c mice revealed the presence of vitreous traction against the internal limiting membrane of the retina which was associated with separation of the nerve fiber layer from the underlying ganglion cell layer. This separation resulted in avulsion of Mueller cell footplates and retinal vessels, hemorrhage into the schisis cavity and disruption of the underlying retinal architecture. The schisis began focally on day 8 or 9 post-inoculation, and the entire retina of most animals was involved by day 10 or 11. These results suggest that retinal schisis involving separation of the nerve fiber layer from the ganglion cell layer contributes to necrosis of the retina in the uninoculated contralateral eye.

AB - Injection of the KOS strain of herpes simplex virus type 1 into the anterior chamber of one eye of a BALB c mouse produces bilateral retinal inflammation which progresses in the contralateral eye to total retinal necrosis by day 10 post-inoculation. Although both immune and direct viral cytopathic effects have been proposed as causes of the initial infiltration, the precise mechanism(s) of the contralateral retinal necrosis remains uncertain. In this study, histopathological examination of the uninoculated eyes of HSV-1 injected BALB c mice revealed the presence of vitreous traction against the internal limiting membrane of the retina which was associated with separation of the nerve fiber layer from the underlying ganglion cell layer. This separation resulted in avulsion of Mueller cell footplates and retinal vessels, hemorrhage into the schisis cavity and disruption of the underlying retinal architecture. The schisis began focally on day 8 or 9 post-inoculation, and the entire retina of most animals was involved by day 10 or 11. These results suggest that retinal schisis involving separation of the nerve fiber layer from the ganglion cell layer contributes to necrosis of the retina in the uninoculated contralateral eye.

KW - herpes simplex virus type 1

KW - retinitis

KW - schisis

KW - vitreoretinal traction

UR - http://www.scopus.com/inward/record.url?scp=0024339201&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024339201&partnerID=8YFLogxK

U2 - 10.1016/0014-4835(89)90061-4

DO - 10.1016/0014-4835(89)90061-4

M3 - Article

C2 - 2731572

AN - SCOPUS:0024339201

VL - 48

SP - 745

EP - 760

JO - Experimental Eye Research

JF - Experimental Eye Research

SN - 0014-4835

IS - 6

ER -