Scalable differentiation and dedifferentiation assays using neuron-free schwann cell cultures

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

This chapter describes protocols to establish simplified in vitro assays of Schwann cell (SC) differentiation in the absence of neurons. The assays are based on the capacity of isolated primary SCs to increase or decrease the expression of myelination-associated genes in response to the presence or absence of cell permeable analogs of cyclic adenosine monophosphate (cAMP). No special conditions of media or substrates beyond the administration or removal of cAMP analogs are required to obtain a synchronous response on differentiation and dedifferentiation. The assays are cost-effective and far easier to implement than traditional myelinating SC-neuron cultures. They are scalable to a variety of plate formats suited for downstream experimentation and analysis. These cell-based assays can be used as drug discovery platforms for the evaluation of novel agents controlling the onset, maintenance, and reversal of the differentiated state using any typical adherent SC population.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages213-232
Number of pages20
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1739
ISSN (Print)1064-3745

Keywords

  • c-Jun
  • cAMP
  • Cell morphology
  • In vitro assays
  • Krox-20
  • MAG
  • Myelin markers
  • O1
  • Primary cultures

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Monje, P. V. (2018). Scalable differentiation and dedifferentiation assays using neuron-free schwann cell cultures. In Methods in Molecular Biology (pp. 213-232). (Methods in Molecular Biology; Vol. 1739). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7649-2_14