Sample preparation and analysis for imaging mass spectrometry

Genea Edwards, Annia Mesa, Robert I. Vazquez-Padron, Jane Marie Kowalski, Sanjoy K. Bhattacharya

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

Imaging mass spectrometry (IMS) is a novel quantitative technique used to investigative diverse biomolecules in tissue sections. Specifically, IMS uses analytical separation of mass spectrometry to determine the spatial distribution of certain lipids and/or proteins located directly on biological sections from a single tissue sample. Typically, IMS is combined with histological analysis to reveal additional distribution details of characterized biomolecules including cell type and/or subcellular localization. In this chapter, we describe the use of Matrix-Assisted Laser Desorption/Ionization (MALDI) Time-Of-Flight/Time-Of-Flight (TOF/TOF) to analyze various cholesterol and phosphatidylcholine species in atherosclerotic plaque of murine heart aortic valves. In particular, we detail animals used, tissue collection, preparation, matrix application, spectra acquisition for generating a color-coded image based on IMS spectral characteristics.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages43-50
Number of pages8
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1609
ISSN (Print)1064-3745

Keywords

  • Biomarker
  • Imaging mass spectrometry (IMS)
  • Matrix-assisted laser desorption ionization (MALDI)
  • Metabolite
  • Time-of-flight (TOF)

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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