Salinity-dependent nickel accumulation and oxidative stress responses in the euryhaline killifish (Fundulus heteroclitus)

Tamzin A. Blewett, Chris M. Wood

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The mechanisms of nickel (Ni) toxicity in marine fish remain unclear, although evidence from freshwater (FW) fish suggests that Ni can act as a pro-oxidant. This study investigated the oxidative stress effects of Ni on the euryhaline killifish (Fundulus heteroclitus) as a function of salinity. Killifish were exposed to sublethal levels (5, 10, and 20 mg L(-1)) of waterborne Ni for 96 h in FW (0 ppt) and 100 % saltwater (SW) (35 ppt). In general, SW was protective against both Ni accumulation and indicators of oxidative stress [protein carbonyl formation and catalase (CAT) activity]. This effect was most pronounced at the highest Ni exposure level. For example, FW intestine showed increased Ni accumulation relative to SW intestine at 20 mg Ni L(-1), and this was accompanied by significantly greater protein carbonylation and CAT activity in this tissue. There were exceptions, however, in that although liver of FW killifish at the highest exposure concentration showed greater Ni accumulation relative to SW liver, levels of CAT activity were greatly decreased. This may relate to tissue- and salinity-specific differences in oxidative stress responses. The results of the present study suggest (1) that there was Ni-induced oxidative stress in killifish, (2) that the effects of salinity depend on differences in the physiology of the fish in FW versus SW, and (3) that increased levels of cations (sodium, calcium, potassium, and magnesium) and anions (SO4 and Cl) in SW are likely protective against Ni accumulation in tissues exposed to the aquatic environment.

Original languageEnglish (US)
Pages (from-to)382-394
Number of pages13
JournalArchives of Environmental Contamination and Toxicology
Volume68
Issue number2
DOIs
StatePublished - Feb 1 2015

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Fundulidae
Oxidative stress
Salinity
Nickel
Oxidative Stress
Fresh Water
Protein Carbonylation
Catalase
Fish
Fishes
Tissue
Liver
Intestines
Proteins
Carbonylation
Physiology
Heat-Shock Proteins
Oxidants
Magnesium
Anions

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Salinity-dependent nickel accumulation and oxidative stress responses in the euryhaline killifish (Fundulus heteroclitus). / Blewett, Tamzin A.; Wood, Chris M.

In: Archives of Environmental Contamination and Toxicology, Vol. 68, No. 2, 01.02.2015, p. 382-394.

Research output: Contribution to journalArticle

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