Salicylate Effects on Proton Gradient Dissipation by Isolated Gastric Mucosal Surface Cells1

Edward J. Olender, Dan Woods, Robert Kozol, David Fromm

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


The effects of salicylate were examined on Na+/H+ exchange by isolated gastric mucosal surface cells loaded with H+ and resuspended in a buffered medium. Choline salicylate (pH 7.4) increases the dissipation of an intracellular proton gradient which was measured using acridine orange. The exchange of extracellular Na+ with intracellular H+ by surface cells not only remains intact but also is enhanced upon exposure to salicylate. This was confirmed by cellular uptake of 22Na and titration of cellular H+ efflux. Salicylate increases Na+/H+ exchange via a pathway predominantly sensitive to amiloride. However, the data also suggest that salicylate dissipates an intracellular proton gradient by an additional mechanism. The latter is independent of extracellular Na+ and not due to a generalized increase in cellular permeability.

Original languageEnglish (US)
Pages (from-to)177-185
Number of pages9
JournalProceedings of the Society for Experimental Biology and Medicine
Issue number2
StatePublished - Nov 1986
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


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