Safety of Autologous Human Schwann Cell Transplantation in Subacute Thoracic Spinal Cord Injury

Kimberly D Anderson, James D Guest, W. Dalton Dietrich, Mary B Bunge, Rosie Curiel Cid, Marine Dididze, Barth A Green, Aisha Khan, Damien D Pearse, Efrat Saraf Lavi, Eva Widerstrom-Noga, Patrick Wood, Allan D Levi

Research output: Contribution to journalArticle

63 Scopus citations

Abstract

The rationale for implantation of autologous human Schwann cells (SCs) in persons with subacute spinal cord injury (SCI) is based on evidence that transplanted SCs are neuroprotective, support local axonal plasticity, and are capable of myelinating axons. A Phase I clinical trial was conducted to evaluate the safety of autologous human SC transplantation into the injury epicenter of six subjects with subacute SCI. The trial was an open-label, unblinded, non-randomized, non-placebo controlled study with a dose escalation design and standard medical rehabilitation. Participants were paraplegics with neurologically complete, trauma-induced spinal lesions. Autologous SCs were cultured in vitro from a sural nerve harvested from each participant and injected into the epicenter of the spinal lesion. Outcome measures for safety were protocol compliance, feasibility, adverse events, stability of neurological level, absence of detectable mass lesion, and the emergence of clinically significant neuropathic pain or muscle spasticity no greater than expected for a natural course cohort. One year post-transplantation, there were no surgical, medical, or neurological complications to indicate that the timing or procedure for the cell transplantation was unsafe. There were no adverse events or serious adverse events related to the cell therapy. There was no evidence of additional spinal cord damage, mass lesion, or syrinx formation. We conclude that it is feasible to identify eligible candidates, appropriately obtain informed consent, perform a peripheral nerve harvest to obtain SCs within 5-30 days of injury, and perform an intra-spinal transplantation of highly purified autologous SCs within 4-7 weeks of injury.

Original languageEnglish (US)
Pages (from-to)2950-2963
Number of pages14
JournalJournal of Neurotrauma
Volume34
Issue number21
DOIs
StatePublished - Nov 1 2017

Keywords

  • autologous transplantation
  • human
  • paraplegia
  • Schwann cells
  • spinal cord injury

ASJC Scopus subject areas

  • Clinical Neurology

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