Saccharomyces cerevisiae cells secreting an aspergillus niger β-galactosidase grow on whey permeate

Vijay Kumar, Sundaram Ramakrishnan, Tuula T. Teeri, Jonathan K.C. Knowles, Brian S. Hartley

Research output: Contribution to journalArticle

70 Scopus citations


We describe the construction of a lactose-utilizing Saccharomyces cerevisiae that expresses the cDNA for a secreted, thermostable β-galactosidase (lacA) from Aspergillus niger. Yeast cells expressing the lacA gene from the yeast ADH1 promoter on a multicopy plasmid secrete up to 40% of the total β-galactosidase activity into the growth medium. The secreted product is extensively N-glycosylated, and cells expressing the lacA gene grow on whey permeate (4% w/v lactose) with a doubling time of 1.6 hours. Such strains may offer a solution to the increasing problem of waste whey disposal.

Original languageEnglish (US)
Pages (from-to)82-85
Number of pages4
JournalNature Biotechnology
Issue number1
StatePublished - Jan 1 1992
Externally publishedYes


ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

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