Saccharomyces cerevisiae cells secreting an aspergillus niger β-galactosidase grow on whey permeate

Vijay Kumar, Sundaram Ramakrishnan, Tuula T. Teeri, Jonathan K.C. Knowles, Brian S. Hartley

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

We describe the construction of a lactose-utilizing Saccharomyces cerevisiae that expresses the cDNA for a secreted, thermostable β-galactosidase (lacA) from Aspergillus niger. Yeast cells expressing the lacA gene from the yeast ADH1 promoter on a multicopy plasmid secrete up to 40% of the total β-galactosidase activity into the growth medium. The secreted product is extensively N-glycosylated, and cells expressing the lacA gene grow on whey permeate (4% w/v lactose) with a doubling time of 1.6 hours. Such strains may offer a solution to the increasing problem of waste whey disposal.

Original languageEnglish (US)
Pages (from-to)82-85
Number of pages4
JournalNature Biotechnology
Volume10
Issue number1
DOIs
StatePublished - Jan 1 1992
Externally publishedYes

Fingerprint

Galactosidases
Aspergillus niger
Aspergillus
Lactose
Yeast
Saccharomyces cerevisiae
Yeasts
Genes
Cells
Plasmids
Complementary DNA
Waste disposal
Growth
Whey

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

Cite this

Saccharomyces cerevisiae cells secreting an aspergillus niger β-galactosidase grow on whey permeate. / Kumar, Vijay; Ramakrishnan, Sundaram; Teeri, Tuula T.; Knowles, Jonathan K.C.; Hartley, Brian S.

In: Nature Biotechnology, Vol. 10, No. 1, 01.01.1992, p. 82-85.

Research output: Contribution to journalArticle

Kumar, Vijay ; Ramakrishnan, Sundaram ; Teeri, Tuula T. ; Knowles, Jonathan K.C. ; Hartley, Brian S. / Saccharomyces cerevisiae cells secreting an aspergillus niger β-galactosidase grow on whey permeate. In: Nature Biotechnology. 1992 ; Vol. 10, No. 1. pp. 82-85.
@article{5b04863315704dcc8e545b32a1f3e873,
title = "Saccharomyces cerevisiae cells secreting an aspergillus niger β-galactosidase grow on whey permeate",
abstract = "We describe the construction of a lactose-utilizing Saccharomyces cerevisiae that expresses the cDNA for a secreted, thermostable β-galactosidase (lacA) from Aspergillus niger. Yeast cells expressing the lacA gene from the yeast ADH1 promoter on a multicopy plasmid secrete up to 40{\%} of the total β-galactosidase activity into the growth medium. The secreted product is extensively N-glycosylated, and cells expressing the lacA gene grow on whey permeate (4{\%} w/v lactose) with a doubling time of 1.6 hours. Such strains may offer a solution to the increasing problem of waste whey disposal.",
author = "Vijay Kumar and Sundaram Ramakrishnan and Teeri, {Tuula T.} and Knowles, {Jonathan K.C.} and Hartley, {Brian S.}",
year = "1992",
month = "1",
day = "1",
doi = "10.1038/nbt0192-82",
language = "English (US)",
volume = "10",
pages = "82--85",
journal = "Nature Biotechnology",
issn = "1087-0156",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - Saccharomyces cerevisiae cells secreting an aspergillus niger β-galactosidase grow on whey permeate

AU - Kumar, Vijay

AU - Ramakrishnan, Sundaram

AU - Teeri, Tuula T.

AU - Knowles, Jonathan K.C.

AU - Hartley, Brian S.

PY - 1992/1/1

Y1 - 1992/1/1

N2 - We describe the construction of a lactose-utilizing Saccharomyces cerevisiae that expresses the cDNA for a secreted, thermostable β-galactosidase (lacA) from Aspergillus niger. Yeast cells expressing the lacA gene from the yeast ADH1 promoter on a multicopy plasmid secrete up to 40% of the total β-galactosidase activity into the growth medium. The secreted product is extensively N-glycosylated, and cells expressing the lacA gene grow on whey permeate (4% w/v lactose) with a doubling time of 1.6 hours. Such strains may offer a solution to the increasing problem of waste whey disposal.

AB - We describe the construction of a lactose-utilizing Saccharomyces cerevisiae that expresses the cDNA for a secreted, thermostable β-galactosidase (lacA) from Aspergillus niger. Yeast cells expressing the lacA gene from the yeast ADH1 promoter on a multicopy plasmid secrete up to 40% of the total β-galactosidase activity into the growth medium. The secreted product is extensively N-glycosylated, and cells expressing the lacA gene grow on whey permeate (4% w/v lactose) with a doubling time of 1.6 hours. Such strains may offer a solution to the increasing problem of waste whey disposal.

UR - http://www.scopus.com/inward/record.url?scp=14744287752&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=14744287752&partnerID=8YFLogxK

U2 - 10.1038/nbt0192-82

DO - 10.1038/nbt0192-82

M3 - Article

C2 - 1368193

AN - SCOPUS:14744287752

VL - 10

SP - 82

EP - 85

JO - Nature Biotechnology

JF - Nature Biotechnology

SN - 1087-0156

IS - 1

ER -