S-nitrosoglutathione modulates CXCR4 and ICOS expression

Yoshihiko Yamamoto, Rajendra Pahwa, Savita Pahwa

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

The expression of CXCR4, a membrane protein which is involved in the entry of HIV-1, is down-modulated from the cell surface by Phorbol 12-myristate 13-acetate (PMA) and the Ca+ ionophore, Ionomycin. Inducible co-stimulator (ICOS), which contributes to lymphocyte proliferation, is up-regulated by PMA/Ionomycin. We examined the influence of S-nitrosoglutathione (SNG), an inhibitor of Vacuolar H+-ATPase (V-ATPase), on the expression of CXCR4 and ICOS in PMA/Ionomycin-treated peripheral mononuclear cells (PBMC), and of CXCR4 alone in lymphoid cell lines. In this report, we show that SNG interferes with both effects of PMA/Ionomycin, namely CXCR4 down-regulation and ICOS up-regulation. These studies imply opposing roles of V-ATPase in the regulation of CXCR4 and ICOS. The influence of SNG in modulating the susceptibility of T cells to HIV-1 and on their immune responses needs further investigation.

Original languageEnglish (US)
Pages (from-to)30-36
Number of pages7
JournalCellular and Molecular Biology Letters
Volume11
Issue number1
DOIs
StatePublished - 2006

Keywords

  • CXCR4
  • HIV-1
  • ICOS
  • S-nitrosoglutathione (SNG)

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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