RYBP and Cbx7 Define Specific Biological Functions of Polycomb Complexes in Mouse Embryonic Stem Cells

LLuis Morey, Luigi Aloia, Luca Cozzuto, Salvador Aznar Benitah, Luciano Di Croce

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

The Polycomb repressive complex 1 (PRC1) is required for decisions of stem cell fate. In mouse embryonic stem cells (ESCs), two major variations of PRC1 complex, defined by the mutually exclusive presence of Cbx7 or RYBP, have been identified. Here, we show that although the genomic localization of the Cbx7- and RYBP-containing PRC1 complexes overlaps in certain genes, it can also be mutually exclusive. At the molecular level, Cbx7 is necessary for recruitment of Ring1B to chromatin, whereas RYBP enhances the PRC1 enzymatic activity. Genes occupied by RYBP show lower levels of Ring1B and H2AK119ub and are consequently more highly transcribed than those bound by Cbx7. At the functional level, we show that genes occupied by RYBP are primarily involved in the regulation of metabolism and cell-cycle progression, whereas those bound by Cbx7 predominantly control early-lineage commitment of ESCs. Altogether, our results indicate that different PRC1 subtypes establish a complex pattern of gene regulation that regulates common and nonoverlapping aspects of ESC pluripotency and differentiation.

Original languageEnglish (US)
Pages (from-to)60-69
Number of pages10
JournalCell Reports
Volume3
Issue number1
DOIs
StatePublished - Jan 31 2013
Externally publishedYes

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Polycomb Repressive Complex 1
Stem cells
Genes
Embryonic Stem Cells
Metabolism
Gene expression
Chromatin
Cell Differentiation
Cell Cycle
Stem Cells
Cells
Mouse Embryonic Stem Cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

RYBP and Cbx7 Define Specific Biological Functions of Polycomb Complexes in Mouse Embryonic Stem Cells. / Morey, LLuis; Aloia, Luigi; Cozzuto, Luca; Benitah, Salvador Aznar; Di Croce, Luciano.

In: Cell Reports, Vol. 3, No. 1, 31.01.2013, p. 60-69.

Research output: Contribution to journalArticle

Morey, LLuis ; Aloia, Luigi ; Cozzuto, Luca ; Benitah, Salvador Aznar ; Di Croce, Luciano. / RYBP and Cbx7 Define Specific Biological Functions of Polycomb Complexes in Mouse Embryonic Stem Cells. In: Cell Reports. 2013 ; Vol. 3, No. 1. pp. 60-69.
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