TY - JOUR
T1 - RX871024 induces Ca2+ mobilization from thapsigargin-sensitive stores in mouse pancreatic β-cells
AU - Efanova, Ioulia B.
AU - Zaitsev, Sergei V.
AU - Brown, Graham
AU - Berggren, Per Olof
AU - Efendić, Suad
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 1998
Y1 - 1998
N2 - The effects of RX871024, a compound with an imidazoline structure, on cytoplasmic-free Ca2+ concentration ([Ca2+](i)) in mouse pancreatic β- cells were studied. RX871024 modulates [Ca2+](i) by at least two mechanisms. One mechanism involves closure of ATP-regulated K+ channels, resulting in membrane depolarization, opening of voltage-gated L-type Ca2+ channels, and a subsequent increase in [Ca2+](i). Another mechanism, reported here for the first time, deals with RX871024-induced mobilization of Ca2+ from nonmitochondrial thapsigargin-sensitive intracellular stores. Reduced glutathione, inhibitors of cytochrome P-450, and monoaminooxidases A and B blocked this Ca2+ mobilization. It is concluded that the mechanism of RX871024-induced Ca2+ mobilization from intracellular stores involves changes in the oxidation/reduction state of the pancreatic β-cell and may be controlled by cytochrome P-450.
AB - The effects of RX871024, a compound with an imidazoline structure, on cytoplasmic-free Ca2+ concentration ([Ca2+](i)) in mouse pancreatic β- cells were studied. RX871024 modulates [Ca2+](i) by at least two mechanisms. One mechanism involves closure of ATP-regulated K+ channels, resulting in membrane depolarization, opening of voltage-gated L-type Ca2+ channels, and a subsequent increase in [Ca2+](i). Another mechanism, reported here for the first time, deals with RX871024-induced mobilization of Ca2+ from nonmitochondrial thapsigargin-sensitive intracellular stores. Reduced glutathione, inhibitors of cytochrome P-450, and monoaminooxidases A and B blocked this Ca2+ mobilization. It is concluded that the mechanism of RX871024-induced Ca2+ mobilization from intracellular stores involves changes in the oxidation/reduction state of the pancreatic β-cell and may be controlled by cytochrome P-450.
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U2 - 10.2337/diab.47.2.211
DO - 10.2337/diab.47.2.211
M3 - Article
C2 - 9519715
AN - SCOPUS:0031594371
VL - 47
SP - 211
EP - 218
JO - Diabetes
JF - Diabetes
SN - 0012-1797
IS - 2
ER -