rpoE, the gene encoding the second heat-shock sigma factor, σE, in Escherichia coli

Pierre E. Rouvière, Alejandro De Las Peñas, Joan Mecsas, Chi Zen Lu, Kenneth E. Rudd, Carol A. Gross

Research output: Contribution to journalArticle

233 Citations (Scopus)

Abstract

In Escherichia coli, the heat shock response is under the control of two alternative sigma factors: σ32 and σE. The σ32-regulated response is well understood, whereas little is known about that of σE, except that it responds to extracytoplasmic immature outer membrane proteins. To further understand this response, we located the rpoE gene at 55.5′ and analyzed the role of σE. σE is required at high temperature, and controls the transcription of at least 10 genes. Some of these might contribute to the integrity of the cell since ΔrpoE cells are more sensitive to SDS plus EDTA and crystal violet. σE controls its own transcription from a σE-dependent promoter, indicating that rpoE transcription plays a role in the regulation of EσE activity. Indeed, under steady-state conditions, the transcription from this promoter mirrors the levels of EσE activity in the cell. However, it is unlikely that the rapid increase in EσE activity following induction can be accounted for solely by increased transcription of rpoE. Based upon homology arguments, we suggest that a gene encoding a negative regulator of σE activity is located immediately downstream of rpoE and may function as the target of the EσE inducing signal.

Original languageEnglish
Pages (from-to)1032-1042
Number of pages11
JournalEMBO Journal
Volume14
Issue number5
StatePublished - Mar 1 1995
Externally publishedYes

Fingerprint

Gene encoding
Transcription
Escherichia coli
Shock
Hot Temperature
Genes
Gentian Violet
Heat-Shock Response
Edetic Acid
Membrane Proteins
Temperature
sporulation-specific sigma factors

Keywords

  • Heat shock
  • Outer membrane proteins
  • Sigma E
  • Sigma factor
  • Thermotolerance

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Rouvière, P. E., De Las Peñas, A., Mecsas, J., Lu, C. Z., Rudd, K. E., & Gross, C. A. (1995). rpoE, the gene encoding the second heat-shock sigma factor, σE, in Escherichia coli. EMBO Journal, 14(5), 1032-1042.

rpoE, the gene encoding the second heat-shock sigma factor, σE, in Escherichia coli. / Rouvière, Pierre E.; De Las Peñas, Alejandro; Mecsas, Joan; Lu, Chi Zen; Rudd, Kenneth E.; Gross, Carol A.

In: EMBO Journal, Vol. 14, No. 5, 01.03.1995, p. 1032-1042.

Research output: Contribution to journalArticle

Rouvière, PE, De Las Peñas, A, Mecsas, J, Lu, CZ, Rudd, KE & Gross, CA 1995, 'rpoE, the gene encoding the second heat-shock sigma factor, σE, in Escherichia coli', EMBO Journal, vol. 14, no. 5, pp. 1032-1042.
Rouvière PE, De Las Peñas A, Mecsas J, Lu CZ, Rudd KE, Gross CA. rpoE, the gene encoding the second heat-shock sigma factor, σE, in Escherichia coli. EMBO Journal. 1995 Mar 1;14(5):1032-1042.
Rouvière, Pierre E. ; De Las Peñas, Alejandro ; Mecsas, Joan ; Lu, Chi Zen ; Rudd, Kenneth E. ; Gross, Carol A. / rpoE, the gene encoding the second heat-shock sigma factor, σE, in Escherichia coli. In: EMBO Journal. 1995 ; Vol. 14, No. 5. pp. 1032-1042.
@article{2d7d096e027f47f38416929d46da5680,
title = "rpoE, the gene encoding the second heat-shock sigma factor, σE, in Escherichia coli",
abstract = "In Escherichia coli, the heat shock response is under the control of two alternative sigma factors: σ32 and σE. The σ32-regulated response is well understood, whereas little is known about that of σE, except that it responds to extracytoplasmic immature outer membrane proteins. To further understand this response, we located the rpoE gene at 55.5′ and analyzed the role of σE. σE is required at high temperature, and controls the transcription of at least 10 genes. Some of these might contribute to the integrity of the cell since ΔrpoE cells are more sensitive to SDS plus EDTA and crystal violet. σE controls its own transcription from a σE-dependent promoter, indicating that rpoE transcription plays a role in the regulation of EσE activity. Indeed, under steady-state conditions, the transcription from this promoter mirrors the levels of EσE activity in the cell. However, it is unlikely that the rapid increase in EσE activity following induction can be accounted for solely by increased transcription of rpoE. Based upon homology arguments, we suggest that a gene encoding a negative regulator of σE activity is located immediately downstream of rpoE and may function as the target of the EσE inducing signal.",
keywords = "Heat shock, Outer membrane proteins, Sigma E, Sigma factor, Thermotolerance",
author = "Rouvi{\`e}re, {Pierre E.} and {De Las Pe{\~n}as}, Alejandro and Joan Mecsas and Lu, {Chi Zen} and Rudd, {Kenneth E.} and Gross, {Carol A.}",
year = "1995",
month = "3",
day = "1",
language = "English",
volume = "14",
pages = "1032--1042",
journal = "EMBO Journal",
issn = "0261-4189",
publisher = "Nature Publishing Group",
number = "5",

}

TY - JOUR

T1 - rpoE, the gene encoding the second heat-shock sigma factor, σE, in Escherichia coli

AU - Rouvière, Pierre E.

AU - De Las Peñas, Alejandro

AU - Mecsas, Joan

AU - Lu, Chi Zen

AU - Rudd, Kenneth E.

AU - Gross, Carol A.

PY - 1995/3/1

Y1 - 1995/3/1

N2 - In Escherichia coli, the heat shock response is under the control of two alternative sigma factors: σ32 and σE. The σ32-regulated response is well understood, whereas little is known about that of σE, except that it responds to extracytoplasmic immature outer membrane proteins. To further understand this response, we located the rpoE gene at 55.5′ and analyzed the role of σE. σE is required at high temperature, and controls the transcription of at least 10 genes. Some of these might contribute to the integrity of the cell since ΔrpoE cells are more sensitive to SDS plus EDTA and crystal violet. σE controls its own transcription from a σE-dependent promoter, indicating that rpoE transcription plays a role in the regulation of EσE activity. Indeed, under steady-state conditions, the transcription from this promoter mirrors the levels of EσE activity in the cell. However, it is unlikely that the rapid increase in EσE activity following induction can be accounted for solely by increased transcription of rpoE. Based upon homology arguments, we suggest that a gene encoding a negative regulator of σE activity is located immediately downstream of rpoE and may function as the target of the EσE inducing signal.

AB - In Escherichia coli, the heat shock response is under the control of two alternative sigma factors: σ32 and σE. The σ32-regulated response is well understood, whereas little is known about that of σE, except that it responds to extracytoplasmic immature outer membrane proteins. To further understand this response, we located the rpoE gene at 55.5′ and analyzed the role of σE. σE is required at high temperature, and controls the transcription of at least 10 genes. Some of these might contribute to the integrity of the cell since ΔrpoE cells are more sensitive to SDS plus EDTA and crystal violet. σE controls its own transcription from a σE-dependent promoter, indicating that rpoE transcription plays a role in the regulation of EσE activity. Indeed, under steady-state conditions, the transcription from this promoter mirrors the levels of EσE activity in the cell. However, it is unlikely that the rapid increase in EσE activity following induction can be accounted for solely by increased transcription of rpoE. Based upon homology arguments, we suggest that a gene encoding a negative regulator of σE activity is located immediately downstream of rpoE and may function as the target of the EσE inducing signal.

KW - Heat shock

KW - Outer membrane proteins

KW - Sigma E

KW - Sigma factor

KW - Thermotolerance

UR - http://www.scopus.com/inward/record.url?scp=0028907529&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028907529&partnerID=8YFLogxK

M3 - Article

C2 - 7889934

AN - SCOPUS:0028907529

VL - 14

SP - 1032

EP - 1042

JO - EMBO Journal

JF - EMBO Journal

SN - 0261-4189

IS - 5

ER -