Rotational and translational motion of troponin C

Martin C. Moncrieffe, Steven Eaton, Željko Bajzer, Christopher Haydock, James D. Potter, Thomas M. Laue, Franklyn G. Prendergast

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Time resolved fluorescence anisotropy and sedimentation velocity has been used to study the rotational and translational hydrodynamic behavior of two mutants of chicken skeletal troponin C bearing a single tryptophan residue at position 78 or 154 in the metal-free-, metal-bound-, and troponin I peptide (residues 96-116 of troponin I)-ligated states. The fluorescence anisotropy data of both mutants were adequately described by two rotational correlation times, and these are compared with the theoretically expected values based on the rotational diffusion of an idealized dumbbell. These data imply that the motion of the N- and C-terminal domains of troponin C are independent. They also suggest that in the metal-free, calcium-saturated and calcium-saturated troponin I peptide-bound states, troponin C is elongated, having an axial ratio of 4-5. Calcium or magnesium binding to the high affinity sites alone reduces the axial ratio to approximately 3. However, with calcium bound to sites III and IV and in the presence of a 1:1 molar ratio of the troponin I peptide, troponin C is approximately spherical. The metal ion and troponin I peptide-induced length changes in troponin C may play a role in the mechanism by which the regulatory function of troponin C is effected.

Original languageEnglish
Pages (from-to)17464-17470
Number of pages7
JournalJournal of Biological Chemistry
Volume274
Issue number25
DOIs
StatePublished - Jun 18 1999
Externally publishedYes

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Troponin C
Troponin I
Metals
Calcium
Peptides
Fluorescence Polarization
Anisotropy
Bearings (structural)
Fluorescence
Hydrodynamics
Sedimentation
Tryptophan
Magnesium
Metal ions
Chickens
Ions

ASJC Scopus subject areas

  • Biochemistry

Cite this

Moncrieffe, M. C., Eaton, S., Bajzer, Ž., Haydock, C., Potter, J. D., Laue, T. M., & Prendergast, F. G. (1999). Rotational and translational motion of troponin C. Journal of Biological Chemistry, 274(25), 17464-17470. https://doi.org/10.1074/jbc.274.25.17464

Rotational and translational motion of troponin C. / Moncrieffe, Martin C.; Eaton, Steven; Bajzer, Željko; Haydock, Christopher; Potter, James D.; Laue, Thomas M.; Prendergast, Franklyn G.

In: Journal of Biological Chemistry, Vol. 274, No. 25, 18.06.1999, p. 17464-17470.

Research output: Contribution to journalArticle

Moncrieffe, MC, Eaton, S, Bajzer, Ž, Haydock, C, Potter, JD, Laue, TM & Prendergast, FG 1999, 'Rotational and translational motion of troponin C', Journal of Biological Chemistry, vol. 274, no. 25, pp. 17464-17470. https://doi.org/10.1074/jbc.274.25.17464
Moncrieffe MC, Eaton S, Bajzer Ž, Haydock C, Potter JD, Laue TM et al. Rotational and translational motion of troponin C. Journal of Biological Chemistry. 1999 Jun 18;274(25):17464-17470. https://doi.org/10.1074/jbc.274.25.17464
Moncrieffe, Martin C. ; Eaton, Steven ; Bajzer, Željko ; Haydock, Christopher ; Potter, James D. ; Laue, Thomas M. ; Prendergast, Franklyn G. / Rotational and translational motion of troponin C. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 25. pp. 17464-17470.
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