Roles for substance P and gastrin-releasing peptide as neurotransmitters released by primary afferent pruriceptors

Tasuku Akiyama, Mitsutoshi Tominaga, Auva Davoodi, Masaki Nagamine, Kevin Blansit, Alexander Horwitz, Mirela Iodi Carstens, E. Carstens

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Recent studies support roles for neurokinin-1 (NK-1) and gastrin-releasing peptide (GRP) receptor-expressing spinal neurons in itch. We presently investigated expression of substance P (SP) and GRP in pruritogen-responsive primary sensory neurons and roles for these neuropeptides in itch signaling. Responses of dorsal root ganglion (DRG) cells to various pruritogens were observed by calcium imaging. DRG cells were then processed for SP, GRP, and isolectin B-4 (IB4; a marker for nonpeptidergic neurons) immunofluorescence. Of prurito-gen-responsive DRG cells, 11.8 -26.8%, 21.8-40.0%, and 21.4-26.8% were immunopositive for SP, GRP, and IB4, respectively. In behavioral studies, both systemic and intrathecal administration of a NK-1 receptor antagonist significantly attenuated scratching evoked by chloroquine and a protease-activated receptor 2 agonist, SLIGRL, but not histamine, bovine adrenal medulla peptide 8-22 (BAM8-22), or serotonin. Systemic or intrathecal administration of a GRP receptor antagonist attenuated scratching evoked by chloroquine and SLIGRL but not BAM8-22 or histamine. The GRP receptor antagonist enhanced scratching evoked by serotonin. These results indicate that SP and GRP expressed in primary sensory neurons are partially involved as neurotransmitters in histamine-independent itch signaling from the skin to the spinal cord.

Original languageEnglish (US)
Pages (from-to)742-748
Number of pages7
JournalJournal of Neurophysiology
Volume109
Issue number3
DOIs
StatePublished - Feb 11 2013
Externally publishedYes

Fingerprint

Gastrin-Releasing Peptide
Bombesin Receptors
seryl-leucyl-isoleucyl-glycyl-arginyl-leucine
Substance P
Neurotransmitter Agents
Spinal Ganglia
Histamine
Chloroquine
Sensory Receptor Cells
Serotonin
Neurokinin-1 Receptor Antagonists
PAR-2 Receptor
Neurons
Peptides
Neuropeptides
Lectins
Fluorescent Antibody Technique
Spinal Cord
Calcium
Skin

Keywords

  • Dorsal root ganglion cell
  • Itch
  • Mouse
  • Scratching

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology

Cite this

Roles for substance P and gastrin-releasing peptide as neurotransmitters released by primary afferent pruriceptors. / Akiyama, Tasuku; Tominaga, Mitsutoshi; Davoodi, Auva; Nagamine, Masaki; Blansit, Kevin; Horwitz, Alexander; Carstens, Mirela Iodi; Carstens, E.

In: Journal of Neurophysiology, Vol. 109, No. 3, 11.02.2013, p. 742-748.

Research output: Contribution to journalArticle

Akiyama, T, Tominaga, M, Davoodi, A, Nagamine, M, Blansit, K, Horwitz, A, Carstens, MI & Carstens, E 2013, 'Roles for substance P and gastrin-releasing peptide as neurotransmitters released by primary afferent pruriceptors', Journal of Neurophysiology, vol. 109, no. 3, pp. 742-748. https://doi.org/10.1152/jn.00539.2012
Akiyama, Tasuku ; Tominaga, Mitsutoshi ; Davoodi, Auva ; Nagamine, Masaki ; Blansit, Kevin ; Horwitz, Alexander ; Carstens, Mirela Iodi ; Carstens, E. / Roles for substance P and gastrin-releasing peptide as neurotransmitters released by primary afferent pruriceptors. In: Journal of Neurophysiology. 2013 ; Vol. 109, No. 3. pp. 742-748.
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